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Literature summary extracted from

  • DeEknamkul, W.; Suttipanta, N.; Kutchan, T.
    Purification and characterization of deacetylipecoside synthase from Alangium lamarckii Thw. (2000), Phytochemistry, 55, 177-181.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.4 alangimarckine Ki: 0.01 mM Alangium lamarckii
4.3.3.4 dehydroalangimarckine Ki: 0.01 mM Alangium lamarckii
4.3.3.4 additional information not inhibited by dopamine, tyramine, tryptamine, cephaeline, emetine and tubulosine Alangium lamarckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.4 0.7
-
 dopamine with fixed concentration of secologanin of 5 mM Alangium lamarckii
4.3.3.4 0.9
-
 secologanin with fixed concentration of dopamine of 5 mM Alangium lamarckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.4 30000
-
 gel filtration, SDS-PAGE Alangium lamarckii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.4 dopamine + secologanin Alangium lamarckii
-
 (1R)-deacetylipecoside + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.4 Alangium lamarckii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.4
-
 Alangium lamarckii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.4 leaf
-
 Alangium lamarckii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.4 0.078
-
 crude extract Alangium lamarckii
4.3.3.4 43.02
-
 purified enzyme Alangium lamarckii

Storage Stability

EC Number Storage Stability Organism
4.3.3.4 -20 - 4°C, 50% loss of activity within 5 days, complete loss of activity within 7 days Alangium lamarckii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.4 dopamine + secologanin
-
 Alangium lamarckii (1R)-deacetylipecoside + H2O
-
 ?
4.3.3.4 dopamine + secologanin highly specific for dopamine Alangium lamarckii (1R)-deacetylipecoside + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.4 monomer gel filtration, SDS-PAGE Alangium lamarckii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.4 45
-
-
 Alangium lamarckii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.4 7.5
-
-
 Alangium lamarckii