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Literature summary extracted from

  • Pries, F.; Kingma, J.; Krooshof, G.H.; Jeronimus-Stratingh, C.M.; Bruins, A.; Janssen, D.B.
    Histidine 289 is essential for hydrolysis of the alkyl-enzyme intermediate of haloalkane dehalogenase (1995), J. Biol. Chem., 270, 10405-10411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 GJ10 mutant H289Q, expression in Escherichia coli Xanthobacter autotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.5 H289Q GJ10, mutant with 660fold reduced activity with substrate 1,2-dibromoethane Xanthobacter autotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.5 halide GJ10 mutant H289Q blocked by bromide in intermediate state Xanthobacter autotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 4
-
 1,2-Dibromoethane GJ10 Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.5 1,2-dichloroethane + H2O Xanthobacter autotrophicus
-
 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O Xanthobacter autotrophicus GJ10
-
 2-chloroethanol + chloride
-
 ?
3.8.1.5 n-haloalkanes + H2O Xanthobacter autotrophicus broad substrate range primary alcohols + halide
-
 ?
3.8.1.5 n-haloalkanes + H2O Xanthobacter autotrophicus GJ10 broad substrate range primary alcohols + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Xanthobacter autotrophicus
-
 GJ10
-
3.8.1.5 Xanthobacter autotrophicus GJ10
-
 GJ10
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 GJ10 mutant H289Q Xanthobacter autotrophicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.8.1.5 1-haloalkane + H2O = a primary alcohol + halide alpha/beta hydrolase with catalytic triad, i.e. nucleophile (Asp124)-histidine (H289)-acid (Asp260), during catalytic reaction Xanthobacter autotrophicus
a
3.8.1.5 1-haloalkane + H2O = a primary alcohol + halide two-step mechanism involving an ester intermediate covalently bound at Asp124, His 289 is important for hydrolysis Xanthobacter autotrophicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus 2-bromoethanol + bromide
-
 ?
3.8.1.5 1,2-dibromoethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-bromoethanol + bromide
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Xanthobacter autotrophicus GJ10 2-chloroethanol + chloride
-
 ?
3.8.1.5 haloalkane + H2O
-
 Xanthobacter autotrophicus alcohol + halide
-
 ?
3.8.1.5 haloalkane + H2O
-
 Xanthobacter autotrophicus GJ10 alcohol + halide
-
 ?
3.8.1.5 n-haloalkanes + H2O broad substrate range Xanthobacter autotrophicus primary alcohols + halide
-
 ?
3.8.1.5 n-haloalkanes + H2O broad substrate range Xanthobacter autotrophicus GJ10 primary alcohols + halide
-
 ?