Any feedback?
Literature summary extracted from
- Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 (1985), J. Bacteriol., 163, 635-639.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | degradation | simple route of detoxification | Xanthobacter autotrophicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.8.1.5 | HgCl2 | - |
Xanthobacter autotrophicus |  |
| 3.8.1.5 | iodoacetamide | - |
Xanthobacter autotrophicus | |
| 3.8.1.5 | NEM | - |
Xanthobacter autotrophicus | |
| 3.8.1.5 | PCMB | - |
Xanthobacter autotrophicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | 1.1 | - |
1,2-Dichloroethane | - |
Xanthobacter autotrophicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 31000 | - |
gel filtration | Xanthobacter autotrophicus |
| 3.8.1.5 | 36000 | - |
1 * 36000, SDS-PAGE | Xanthobacter autotrophicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.5 | Xanthobacter autotrophicus | - |
GJ10 | - |
| 3.8.1.5 | Xanthobacter autotrophicus GJ10 | - |
GJ10 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | GJ10 | Xanthobacter autotrophicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.8.1.5 | 4°C, 50 mM Tris-sulfate buffer, pH 7.5, 1 mM EDTA, 1 mM beta-mercaptoethanol, less than 20% loss of activity after several weeks | Xanthobacter autotrophicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | 1,2-dibromoethane + H2O | 94% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dichloroethane + H2O | highest activity | Xanthobacter autotrophicus | 2-chloroethanol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dichloroethane + H2O | highest activity | Xanthobacter autotrophicus GJ10 | 2-chloroethanol + chloride | - |
? | |
| 3.8.1.5 | 1,3-dichloropropane + H2O | 80% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | ? | - |
? | |
| 3.8.1.5 | 1-bromopropane + H2O | 29% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | propanol + bromide | - |
? | |
| 3.8.1.5 | 1-chlorobutane + H2O | 31% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | butanol + chloride | - |
? | |
| 3.8.1.5 | 1-chloropropane + H2O | 51% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | propanol + chloride | - |
? | |
| 3.8.1.5 | 1-chloropropane + H2O | 51% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus GJ10 | propanol + chloride | - |
? | |
| 3.8.1.5 | 1-iodopropane + H2O | 14% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | propanol + iodide | - |
? | |
| 3.8.1.5 | 3-chloropropene + H2O | 45% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | propenol + chloride | - |
? | |
| 3.8.1.5 | bromoethane + H2O | 24% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | ethanol + bromide | - |
? | |
| 3.8.1.5 | chloroethane + H2O | 24% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | ethanol + chloride | - |
? | |
| 3.8.1.5 | chloroethane + H2O | 24% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus GJ10 | ethanol + chloride | - |
? | |
| 3.8.1.5 | chloromethane + H2O | 28% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus | methanol + chloride | - |
? | |
| 3.8.1.5 | chloromethane + H2O | 28% of the activity with 1,2-dichloroethane | Xanthobacter autotrophicus GJ10 | methanol + chloride | - |
? | |
| 3.8.1.5 | haloalkane + H2O | n-halogenated C1 to C4 alkanes, including chlorinated, brominated and iodinated compounds | Xanthobacter autotrophicus | alcohol + halide | - |
? | |
| 3.8.1.5 | haloalkane + H2O | n-halogenated C1 to C4 alkanes, including chlorinated, brominated and iodinated compounds | Xanthobacter autotrophicus GJ10 | alcohol + halide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | monomer | 1 * 36000, SDS-PAGE | Xanthobacter autotrophicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 37 | - |
- |
Xanthobacter autotrophicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 50 | - |
inactivation above 50°C | Xanthobacter autotrophicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 8.2 | - |
- |
Xanthobacter autotrophicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
