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Literature summary extracted from

  • Pratt, M.M.
    Purification of cytoplasmic dynein from Strongylocentrotus sea urchin eggs (1986), Methods Enzymol., 134, 325-337.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.2 cytoplasm
-
 Strongylocentrotus purpuratus 5737
-
5.6.1.2 cytoplasm
-
 Strongylocentrotus droebachiensis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.2 Ca2+
-
 Strongylocentrotus purpuratus
5.6.1.2 Ca2+
-
 Strongylocentrotus droebachiensis
5.6.1.2 Mg2+
-
 Strongylocentrotus purpuratus
5.6.1.2 Mg2+
-
 Strongylocentrotus droebachiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.2 320000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
5.6.1.2 320000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis
5.6.1.2 360000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
5.6.1.2 360000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis
5.6.1.2 385000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
5.6.1.2 385000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n Strongylocentrotus purpuratus
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n Strongylocentrotus droebachiensis
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Strongylocentrotus droebachiensis
-
 sea urchin
-
5.6.1.2 Strongylocentrotus purpuratus
-
 sea urchin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.2 ammonium sulfate precipitation, DEAE-Sephacel, Calmodulin-affinity column, 5-20% sucrose gradient centrifugation Strongylocentrotus purpuratus
5.6.1.2 ammonium sulfate precipitation, DEAE-Sephacel, Calmodulin-affinity column, 5-20% sucrose gradient centrifugation Strongylocentrotus droebachiensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.2 egg
-
 Strongylocentrotus purpuratus
-
5.6.1.2 egg
-
 Strongylocentrotus droebachiensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.2 0.2
-
 sucrose gradient pool Strongylocentrotus purpuratus
5.6.1.2 0.2
-
 sucrose gradient pool Strongylocentrotus droebachiensis

Storage Stability

EC Number Storage Stability Organism
5.6.1.2 0°C, buffered solution with protease inhibitors, 5 days, 20%, -80°C, liqid nitrogen, 6 months, 20% Strongylocentrotus purpuratus
5.6.1.2 0°C, buffered solution with protease inhibitors, 5 days, 20%, -80°C, liqid nitrogen, 6 months, 20% Strongylocentrotus droebachiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Strongylocentrotus purpuratus ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Strongylocentrotus droebachiensis ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.1.2 oligomer alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
5.6.1.2 oligomer alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis