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Literature summary extracted from

  • Hisanaga S.I.; Sakai, H.
    Purification of cytoplasmic dynein from sea urchin eggs (1986), Methods Enzymol., 134, 337-351.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.1.2 Calmodulin from porcine brain, 3-to 8fold Hemicentrotus pulcherrimus
5.6.1.2 sea urchin egg 3-to 8fold Hemicentrotus pulcherrimus
5.6.1.2 Triton X-100 3.5fold activation, 0.1% Hemicentrotus pulcherrimus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.2 erythro-9-[3-(2-hydroxynonyl)]adenine half maximal inhibition at 3 to 5 mM Hemicentrotus pulcherrimus
5.6.1.2 VO43- 50% inhibition at 0.001 mM Hemicentrotus pulcherrimus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.2 cytoplasm
-
 Hemicentrotus pulcherrimus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.2 550000
-
 12S sucrose density gradient fraction, calculated from sedimentation constant (12.3 S) and Stokes radius (10.2 nm) Hemicentrotus pulcherrimus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n Hemicentrotus pulcherrimus
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Hemicentrotus pulcherrimus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.2 ammonium sulfate precipitation, Sepharose 4B, hydroxyapatite column, 5-20% sucrose density gradient Hemicentrotus pulcherrimus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.2 egg
-
 Hemicentrotus pulcherrimus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.2 0.8
-
 12S sucrose gradient fraction Hemicentrotus pulcherrimus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Hemicentrotus pulcherrimus ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?