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Literature summary extracted from

  • Kahn, D.W.; Anderson, B.M.
    Characterization of Haemophilus influenzae nucleotide pyrophosphatase. An enzyme of critical importance for growth of the organism (1996), J. Biol. Chem., 261, 6016-6025.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.6.1.9 thermolabile Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 2,3-Butanedione inactivation half-life: 21.8 min at 0.1 mM Haemophilus influenzae
3.6.1.9 5'-ADP Ki: 0.0016 mM Haemophilus influenzae
3.6.1.9 5'-AMP biphasic kinetics abolished in the presence of 0.2 mM, Ki: 0.015 mM Haemophilus influenzae
3.6.1.9 adenosine 5'-sulfate Ki: 0.052 mM Haemophilus influenzae
3.6.1.9 GMP Ki: 0.022 mM Haemophilus influenzae
3.6.1.9 N-ethyl-5-phenylisoxazolium-3'-sulfonate Woodward's reagent, inactivation half-life: 16 min at 0.05 mM Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.003
-
 pyridine adenine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 0.005
-
 NAD+ biphasic hydrolysis, functions in a manner indicative of negative cooperativity Haemophilus influenzae
3.6.1.9 0.006
-
 3-acetylpyridine adenine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 0.008
-
 nicotinic acid adenine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 0.011
-
 1,4-dihydronicotinamide adenine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 0.015
-
 nicotinamide hypoxanthine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 0.02
-
 FAD
-
 Haemophilus influenzae
3.6.1.9 0.026
-
 nicotinamide 1,N6-ethenoadenine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 0.056
-
 3-Aminopyridine adenine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 0.632
-
 3-aminopyridine 1,N6-ethenoadenine dinucleotide
-
 Haemophilus influenzae
3.6.1.9 1.29
-
 3-aminopyridine 1,N6-ethenoadenine dinucleotide
-
 Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 67800
-
 gel filtration Haemophilus influenzae
3.6.1.9 104800 108100 in the presence of 0.1-0.4 mM NAD, gel filtration Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 FAD + H2O Haemophilus influenzae
-
 FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O Haemophilus influenzae
-
 NMN + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Haemophilus influenzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.9 glycoprotein 16% carbohydrate by weight Haemophilus influenzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 to homogeneity, chromatography techniques Haemophilus influenzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 cell culture growth inhibition by AMP, ADP when NAD serves as V-factor Haemophilus influenzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 3500
-
-
 Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 1,4-dihydronicotinamide adenine dinucleotide + H2O
-
 Haemophilus influenzae 1,4-dihydronicotinamide mononucleotide + 5'-AMP
-
 ?
3.6.1.9 3-acetylpyridine adenine dinucleotide + H2O
-
 Haemophilus influenzae 3-acetylpyridine riboside 5'-phosphate + 5'-AMP
-
 ?
3.6.1.9 3-aminopyridine 1,N6-ethenoadenine dinucleotide + H2O
-
 Haemophilus influenzae 3-aminopyridine riboside + N6-etheneadenosine 5'-phosphate
-
 ?
3.6.1.9 3-aminopyridine adenine dinucleotide + H2O
-
 Haemophilus influenzae 3-aminopyridine riboside 5'-phosphate + 5'-AMP
-
 ?
3.6.1.9 FAD + H2O
-
 Haemophilus influenzae FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O
-
 Haemophilus influenzae NMN + AMP
-
 ?
3.6.1.9 nicotinamide 1,N6-ethenoadenine dinucleotide + H2O
-
 Haemophilus influenzae NMN + N6-ethenoadenosine 5'-phosphate
-
 ?
3.6.1.9 nicotinamide guanine dinucleotide + H2O
-
 Haemophilus influenzae NMN + GMP
-
 ?
3.6.1.9 nicotinamide hypoxanthine dinucleotide + H2O
-
 Haemophilus influenzae NMN + hypoxanthine monophosphate
-
 ?
3.6.1.9 nicotinic acid adenine dinucleotide + H2O
-
 Haemophilus influenzae nicotinic acid mononucleotide + 5'-AMP
-
 ?
3.6.1.9 pyridine adenine dinucleotide + H2O
-
 Haemophilus influenzae pyridine riboside 5'-phosphate + 5'-AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.9 monomer 1 * 67800, SDS-PAGE Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 8 9
-
 Haemophilus influenzae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.9 0.022
-
 GMP
-
 Haemophilus influenzae
3.6.1.9 0.052
-
 adenosine 5'-sulfate
-
 Haemophilus influenzae