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Literature summary extracted from

  • Medda, R.; Padiglia, A.; Lorrai, A.; Murgia, B.; Agro, A.F.; Castagnola, M.; Floris, G.
    Purification and properties of a nucleotide pyrophosphatase from lentil seddlings (2000), J. Protein Chem., 19, 209-214.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 Cu2+ 50% inhibition at 1.5 mM Lens culinaris
3.6.1.9 diethyldiphosphate inactivation follows apparent first-order kinetics, completely restored by hydroxylamine Lens culinaris
3.6.1.9 EDTA 50% inhibition at 0.2 mM Lens culinaris
3.6.1.9 Ni2+ 50% inhibition at 2 mM Lens culinaris
3.6.1.9 Zn2+ 50% inhibition at 20 mM Lens culinaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.042
-
 NADH
-
 Lens culinaris
3.6.1.9 0.043
-
 NADP+
-
 Lens culinaris
3.6.1.9 0.047
-
 NADPH
-
 Lens culinaris
3.6.1.9 0.049
-
 NAD+
-
 Lens culinaris
3.6.1.9 0.066
-
 thymidine 5'-monophosphate p-nitrophenyl ester
-
 Lens culinaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.9 Ca2+ activation Lens culinaris
3.6.1.9 Mg2+ activation Lens culinaris
3.6.1.9 Mn2+ 100% activation at 20 mM Lens culinaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 75000
-
 gel filtration Lens culinaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 3 diadenosine 5',5''-P1,P4-tetraphosphate + 3 H2O Lens culinaris
-
 2 ATP + 2 ADP + 2 AMP
-
 ?
3.6.1.9 ADP-ribose + H2O Lens culinaris low activity AMP + phosphoribose
-
 ?
3.6.1.9 alpha-NADH + H2O Lens culinaris low activity alpha-NMNH + AMP
-
 ?
3.6.1.9 diadenosine 5',5''-P1, P2-diphosphate + H2O Lens culinaris
-
 AMP + AMP
-
 ?
3.6.1.9 diadenosine 5',5''-P1,P3-triphosphate + H2O Lens culinaris
-
 AMP + ADP
-
 ?
3.6.1.9 diadenosine 5',5''-P1,P5-pentaposphate + H2O Lens culinaris
-
 ATP + ADP + AMP + adenosine 5'-tetraphosphate
-
 ?
3.6.1.9 FAD + H2O Lens culinaris low activity FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O Lens culinaris
-
 NMN + AMP
-
 ?
3.6.1.9 NADH + H2O Lens culinaris best natural substrate, reduced form more rapidly hydrolysed than oxidized form NMNH + AMP
-
 ?
3.6.1.9 NADP+ + H2O Lens culinaris
-
 NMN + adenosine 2',5'-phosphate
-
 ?
3.6.1.9 NADPH + H2O Lens culinaris reduced form more rapidly hydrolysed than oxidized form NMNH + adenosine 2',5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Lens culinaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 to homogeneity, chromatography techniques Lens culinaris

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.9 a nucleoside triphosphate + H2O = a nucleotide + diphosphate substrates include NAD+, NADP+, FAD, CoA and also ATP and ADP Lens culinaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 seedling
-
 Lens culinaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 16.9
-
-
 Lens culinaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 3 diadenosine 5',5''-P1,P4-tetraphosphate + 3 H2O
-
 Lens culinaris 2 ATP + 2 ADP + 2 AMP
-
 ?
3.6.1.9 ADP-ribose + H2O low activity Lens culinaris AMP + phosphoribose
-
 ?
3.6.1.9 alpha-NADH + H2O low activity Lens culinaris alpha-NMNH + AMP
-
 ?
3.6.1.9 diadenosine 5',5''-P1, P2-diphosphate + H2O
-
 Lens culinaris AMP + AMP
-
 ?
3.6.1.9 diadenosine 5',5''-P1,P3-triphosphate + H2O
-
 Lens culinaris AMP + ADP
-
 ?
3.6.1.9 diadenosine 5',5''-P1,P5-pentaposphate + H2O
-
 Lens culinaris ATP + ADP + AMP + adenosine 5'-tetraphosphate
-
 ?
3.6.1.9 FAD + H2O low activity Lens culinaris FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O
-
 Lens culinaris NMN + AMP
-
 ?
3.6.1.9 NADH + H2O best natural substrate, reduced form more rapidly hydrolysed than oxidized form Lens culinaris NMNH + AMP
-
 ?
3.6.1.9 NADP+ + H2O
-
 Lens culinaris NMN + adenosine 2',5'-phosphate
-
 ?
3.6.1.9 NADPH + H2O reduced form more rapidly hydrolysed than oxidized form Lens culinaris NMNH + adenosine 2',5'-phosphate
-
 ?
3.6.1.9 P1-5'-(7-methyl)-guanosine-P3-5'-guanosine triphosphate + H2O cap analogue Lens culinaris 7-methylguanosine 5'-diphosphate + GMP
-
 ?
3.6.1.9 thymidine 5'-monophosphate p-nitrophenyl ester + H2O faster cleavage than other substrates tested Lens culinaris thymidine 5'-monophosphate + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.9 monomer 1 * 75000, SDS-PAGE Lens culinaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 10
-
-
 Lens culinaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.9 0.2
-
 EDTA
-
 Lens culinaris
3.6.1.9 1.5
-
 Cu2+
-
 Lens culinaris
3.6.1.9 2
-
 Ni2+
-
 Lens culinaris
3.6.1.9 20
-
 Zn2+
-
 Lens culinaris