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Literature summary extracted from

  • Ikehata, H.
    The purification of thiaminase II (1960), J. Gen. Appl. Microbiol., 6, 30-39.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.99.2 EDTA in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus
3.5.99.2 L-cysteine in a concentration range of 0.02 mM to 1000 mM Aneurinibacillus aneurinilyticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.99.2 dialyzing the purified enzyme solution against 52% saturated ammonium sulfate solution, two weeks Aneurinibacillus aneurinilyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.99.2 As2O3 0.01 mM 33% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Ca2+ 0.01 mM 45% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Cd2+ 0.01 mM 88% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Cu2+ 0.01 mM 100% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Fe2+ 0.01 mM 100% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Fe3+ 0.01 mM 45% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 ICH2COOH 0.01 mM 42% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Li+ 0.01 mM 30% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Mg2+ 0.01 mM 30% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Mn2+ 0.01 mM 30% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Ni2+ 0.01 mM 73% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 p-chloromercuribenzoate 0.01 mM 99% inhibition Aneurinibacillus aneurinilyticus
3.5.99.2 Zn2+ 0.01 mM 100% inhibition Aneurinibacillus aneurinilyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.2 0.003
-
 thiamine
-
 Aneurinibacillus aneurinilyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.2 thiamine + H2O Aneurinibacillus aneurinilyticus
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.2 Aneurinibacillus aneurinilyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.2
-
 Aneurinibacillus aneurinilyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.2 thiamine + H2O
-
 Aneurinibacillus aneurinilyticus 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.99.2 8.6
-
-
 Aneurinibacillus aneurinilyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.99.2 6.6 9.4
-
 Aneurinibacillus aneurinilyticus