Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Klein, A.R.; Breitung, J.; Linder, D.; Stetter, K.O.; Thauer, R.K.
    N5,N10-Methenyltetrahydromethanopterin cyclohydrolase from the extremely thermophilic sulfate reducing Archaeoglobus fulgidus: comparison of its properties with those of the cyclohydrolase from the extremely thermophilic Methanopyrus kandleri (1993), Arch. Microbiol., 159, 213-219.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.27 0.22
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin
-
 Archaeoglobus fulgidus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.27 cytoplasm
-
 Archaeoglobus fulgidus 5737
-
3.5.4.27 cytoplasm
-
 Methanopyrus kandleri 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.27 K2HPO4 1.5 M increases the activity 1.6fold Archaeoglobus fulgidus
3.5.4.27 K2HPO4 1.5 M K2HPO4 required for optimal activity Methanopyrus kandleri
3.5.4.27 Na2SO4 1 M, can substitute for K2HPO4 in stimulation Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.27 39000
-
 2 * 39000, SDS-PAGE Archaeoglobus fulgidus
3.5.4.27 80000
-
 non-denaturing PAGE Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.27 Archaeoglobus fulgidus
-
-
-
3.5.4.27 Methanopyrus kandleri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.27
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.27 additional information
-
-
 Archaeoglobus fulgidus
3.5.4.27 additional information
-
-
 Methanopyrus kandleri
3.5.4.27 1765
-
-
 Archaeoglobus fulgidus

Storage Stability

EC Number Storage Stability Organism
3.5.4.27 -20°C, stable for several weeks Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Archaeoglobus fulgidus N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanopyrus kandleri N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.27 dimer 2 * 39000, SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.27 85
-
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.27 additional information
-
 no requirement of salts for thermostability Methanopyrus kandleri
3.5.4.27 90
-
-
 Methanopyrus kandleri
3.5.4.27 90
-
 more than 80% loss of activity after 10 min without stabilizer, completely stable for more than 50 min with addition of 1.0 M K2HPO4 Archaeoglobus fulgidus
3.5.4.27 90
-
 1.5 M K2HPO4 is required for optimal stability Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.27 8.5
-
-
 Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.27 additional information
-
-
 Methanopyrus kandleri