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Literature summary extracted from
- Allantoinase from Pseudomonas aeruginosa (1982), Biochim. Biophys. Acta, 718, 212-219.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.5 | Cu2+ | - |
Pseudomonas aeruginosa |  |
| 3.5.2.5 | Hg2+ | - |
Pseudomonas aeruginosa | |
| 3.5.2.5 | N-ethylmaleimide | - |
Pseudomonas aeruginosa | |
| 3.5.2.5 | p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.2.5 | 5 | - |
allantoin | - |
Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.5 | 38000 | - |
4 * 38000, SDS-PAGE | Pseudomonas aeruginosa |
| 3.5.2.5 | 140000 | 150000 | gel filtration | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.5 | allantoin + H2O | Pseudomonas aeruginosa | - |
allantoate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.5 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.2.5 | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.5 | 0.99 | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.2.5 | 4°C, 40 mM phosphate buffer, pH 7, 0.01% sodium azide, 1 mM beta-mercaptoethanol, 4 months | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.5 | allantoin + H2O | - |
Pseudomonas aeruginosa | allantoate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.2.5 | tetramer | 4 * 38000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.5 | 50 | - |
rapidly inactivitad above 50°C | Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.5 | 8.4 | - |
- |
Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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