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Literature summary extracted from

  • Ogawa, J.; Shimizu, S.
    Purification and characterization of dihydroorotase from Pseudomonas putida (1995), Arch. Microbiol., 164, 353-357.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.3 N-carbamoylamino acids competitive inhibition Pseudomonas putida
3.5.2.3 Orotate noncompetitive inhibition Pseudomonas putida
3.5.2.3 sulfhydryl reagents
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.3 0.081
-
 L-dihydroorotate
-
 Pseudomonas putida
3.5.2.3 2.2
-
 L-carbamoylaspartate
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.3 41000
-
 2 * 41000 SDS-PAGE Pseudomonas putida
3.5.2.3 82000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.3 N-carbamoyl-L-aspartate Pseudomonas putida
-
 L-dihydroorotate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.3 18
-
 L-dihydroorotate as substrate Pseudomonas putida
3.5.2.3 68
-
 L-carbamoylaspartate as substrate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 L-carbamoylaspartate
-
 Pseudomonas putida L-dihydroorotate + H2O
-
 r
3.5.2.3 N-carbamoyl-L-aspartate
-
 Pseudomonas putida L-dihydroorotate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.2.3 dimer 2 * 41000 SDS-PAGE Pseudomonas putida