Literature summary extracted from

Krungkrai, J.; Cerami, A.; Henderson, G.B.
Pyrimidine biosynthesis in parasitic protozoa: purification of a monofunctional dihydroorotase from Plasmodium berghei and Crithidia fasciculata (1990), Biochemistry, 29, 6270-6275.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.5.2.3	Dimethylsulfoxide	increases the catalytic efficiency on the ring cyclization reaction, but not on the ring hydrolysis	Crithidia fasciculata
3.5.2.3	Dimethylsulfoxide	increases the catalytic efficiency on the ring cyclization reaction, but not on the ring hydrolysis	Plasmodium berghei

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.5.2.3	diethyldicarbonate	-	Crithidia fasciculata
3.5.2.3	diethyldicarbonate	-	Plasmodium berghei

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.5.2.3	0.026	-	L-dihydroorotate	-	Crithidia fasciculata
3.5.2.3	0.026	-	L-dihydroorotate	-	Plasmodium berghei
3.5.2.3	0.846	-	L-carbamoylaspartate	-	Crithidia fasciculata
3.5.2.3	0.846	-	L-carbamoylaspartate	-	Plasmodium berghei

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.2.3	38000	-	1 * 38000, SDS-PAGE	Crithidia fasciculata
3.5.2.3	38000	-	1 * 38000, SDS-PAGE	Plasmodium berghei
3.5.2.3	40000	-	gel filtration	Plasmodium berghei
3.5.2.3	42000	-	gel filtration	Crithidia fasciculata
3.5.2.3	44000	-	1 * 44000, SDS-PAGE	Crithidia fasciculata

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.5.2.3	N-carbamoyl-L-aspartate	Crithidia fasciculata	-	L-dihydroorotate + H2O	-	r
3.5.2.3	N-carbamoyl-L-aspartate	Plasmodium berghei	-	L-dihydroorotate + H2O	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.2.3	Crithidia fasciculata	-	-	-
3.5.2.3	Plasmodium berghei	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.2.3	-	Crithidia fasciculata
3.5.2.3	-	Plasmodium berghei

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.2.3	L-carbamoylaspartate	-	Crithidia fasciculata	L-dihydroorotate + H2O	-	r
3.5.2.3	L-carbamoylaspartate	-	Plasmodium berghei	L-dihydroorotate + H2O	-	r
3.5.2.3	N-carbamoyl-L-aspartate	-	Crithidia fasciculata	L-dihydroorotate + H2O	-	r
3.5.2.3	N-carbamoyl-L-aspartate	-	Plasmodium berghei	L-dihydroorotate + H2O	-	r

Subunits

EC Number	Subunits	Comment	Organism
3.5.2.3	monomer	1 * 38000, SDS-PAGE	Crithidia fasciculata
3.5.2.3	monomer	1 * 38000, SDS-PAGE	Plasmodium berghei
3.5.2.3	monomer	1 * 44000, SDS-PAGE	Crithidia fasciculata

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.5.2.3	0.183	-	L-dihydroorotate	-	Plasmodium berghei
3.5.2.3	0.653	-	L-carbamoylaspartate	-	Crithidia fasciculata
3.5.2.3	0.653	-	L-carbamoylaspartate	-	Plasmodium berghei
3.5.2.3	4.31	-	L-dihydroorotate	-	Crithidia fasciculata
3.5.2.3	4.31	-	L-dihydroorotate	-	Plasmodium berghei

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Release 2023.1 (January 2023)