Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • La Nauze, J.M.; Rosenberg, H.; Shaw, D.C.
    The enzyme cleavage of the carbon-phosphorous bond: purification and properties of phosphonatase (1970), Biochim. Biophys. Acta, 212, 332-350.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.11.1.1 inactivation in absence of Mg2+ is aggravated at higher pH or when either EDTA or Ca2+ is added Bacillus cereus
3.11.1.1 repeated freezing and thawing causes rapid loss of activity Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.11.1.1 CN-
-
 Bacillus cereus
3.11.1.1 NaCl
-
 Bacillus cereus
3.11.1.1 phosphite
-
 Bacillus cereus
3.11.1.1 S2-
-
 Bacillus cereus
3.11.1.1 SO32-
-
 Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.11.1.1 0.04
-
 phosphonoacetaldehyde
-
 Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.11.1.1 copper contains 0.3 gatom per molecular weight of 75000 Da Bacillus cereus
3.11.1.1 Iron contains trace amounts of iron Bacillus cereus
3.11.1.1 Mg2+ required Bacillus cereus
3.11.1.1 Zinc contains 0.4 gatom per molecular weight of 75000 Da Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.11.1.1 68000
-
 nondenaturing PAGE Bacillus cereus
3.11.1.1 83000
-
 gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.11.1.1 phosphonoacetylaldehyde + H2O Bacillus cereus second step in the pathway by which Bacillus cereus metabolizes 2-aminoethylphosphonic acid acetaldehyde + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.11.1.1 Bacillus cereus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.11.1.1 13.9
-
-
 Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
3.11.1.1 -20°C, stable for several months Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.11.1.1 p-nitrophenylphosphate + H2O hydrolyzed at considerable lower rate than phosphonoacetaldehyde Bacillus cereus p-nitrophenol + phosphate
-
 ?
3.11.1.1 phosphonoacetylaldehyde + H2O 2-phosphonoacetaldehyde Bacillus cereus acetaldehyde + phosphate
-
 ?
3.11.1.1 phosphonoacetylaldehyde + H2O second step in the pathway by which Bacillus cereus metabolizes 2-aminoethylphosphonic acid Bacillus cereus acetaldehyde + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.11.1.1 dimer 2 * 33000-37000, similar subunits, SDS-PAGE Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.11.1.1 45
-
-
 Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.11.1.1 30 55 30°C: about 50% of maximal activity, 55°C: about 45% of maximal activity Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.11.1.1 35
-
 rapid loss loss of activity in absence of Mg2+ Bacillus cereus
3.11.1.1 45
-
 heat-labile, even in presence of 5 mM Mg2+ Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.11.1.1 16.7
-
 2-phosphonoacetaldehyde
-
 Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.11.1.1 8 9
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.11.1.1 6.5 10 pH 6.5: about 35% of maximal activity, pH 10.0: about 30% of maximal activity Bacillus cereus