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Literature summary extracted from

  • Sylvestre, M.; Hurtubise, Y.; Barriault, D.; Bergeron, J.; Ahmad, D.
    Characterization of active recombinant 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni B-356 and sequence of the encoding gene (bphB) (1996), Appl. Environ. Microbiol., 62, 2710-2715.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.56 0.073
-
 2,3-dihydro-2,3,-dihydroxybiphenyl activity measured with 1 micromol NAD+ in the reaction mixture at pH 9.5 and 37°C Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.56 123000
-
 native protein, gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.56 cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ Comamonas testosteroni biphenyl degradation pathway 2,3-dihydroxybiphenyl + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.56 Comamonas testosteroni
-
 gene bphB
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.56 46
-
-
 Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
1.3.1.56 -70°C for months Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.56 cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+
-
 Comamonas testosteroni 2,3-dihydroxybiphenyl + NADH
-
 ?
1.3.1.56 cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD+ biphenyl degradation pathway Comamonas testosteroni 2,3-dihydroxybiphenyl + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.56 tetramer
-
 Comamonas testosteroni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.56 55
-
-
 Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.56 9.5
-
 recombinant His-tagged enzyme Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.56 NAD+ essential cofactor for His-taggged enzyme Comamonas testosteroni