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Literature summary extracted from
- Purification and properties of D-xylulose reductase from Pachysolen tannophilus (1986), J. Ferment. Technol., 64, 219-225.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | cysteine | slight activation | Pachysolen tannophilus |  |
| 1.1.1.9 | glycine | slight activation | Pachysolen tannophilus | |
| 1.1.1.9 | GSH | activation | Pachysolen tannophilus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.9 | freezing/thawing inactivates, 8 cycles lead to 89% loss of activity and 11 cycles to complete inactivation | Pachysolen tannophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | AgNO3 | - |
Pachysolen tannophilus | |
| 1.1.1.9 | CuSO4 | - |
Pachysolen tannophilus | |
| 1.1.1.9 | cysteine | at 294 mM | Pachysolen tannophilus | |
| 1.1.1.9 | FeSO4 | - |
Pachysolen tannophilus | |
| 1.1.1.9 | HgCl2 | complete inhibition | Pachysolen tannophilus | |
| 1.1.1.9 | iodoacetate | complete inhibition | Pachysolen tannophilus | |
| 1.1.1.9 | MnSO4 | - |
Pachysolen tannophilus | |
| 1.1.1.9 | NaCl | at 160 mM | Pachysolen tannophilus | |
| 1.1.1.9 | NH4Cl | at 310 mM | Pachysolen tannophilus | |
| 1.1.1.9 | Pb(CH3COO)2 | - |
Pachysolen tannophilus | |
| 1.1.1.9 | ZnSO4 | complete inhibition | Pachysolen tannophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.9 | 11 | - |
xylitol | - |
Pachysolen tannophilus | |
| 1.1.1.9 | 30 | - |
D-sorbitol | - |
Pachysolen tannophilus | |
| 1.1.1.9 | 50 | - |
ribitol | - |
Pachysolen tannophilus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.9 | cytoplasm | - |
Pachysolen tannophilus | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | KCl | slight activation | Pachysolen tannophilus | |
| 1.1.1.9 | Mg2+ | - |
Pachysolen tannophilus | |
| 1.1.1.9 | NaCl | activation | Pachysolen tannophilus | |
| 1.1.1.9 | NH4Cl | activation | Pachysolen tannophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 40000 | - |
4 * 40000, SDS-PAGE | Pachysolen tannophilus |
| 1.1.1.9 | 120000 | - |
gel filtration | Pachysolen tannophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.9 | Pachysolen tannophilus | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 29.9 | - |
- |
Pachysolen tannophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.9 | -15°C, 60% loss of activity after 1 month | Pachysolen tannophilus |
| 1.1.1.9 | -2°C, purified stable for a month | Pachysolen tannophilus |
| 1.1.1.9 | 0°C, 34% loss of activity within a month | Pachysolen tannophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.9 | D-ribitol + NAD+ | 85% of the rate of xylitol oxidation | Pachysolen tannophilus | D-ribulose + NADH | - |
? | |
| 1.1.1.9 | D-sorbitol + NAD+ | rate of xylitol oxidation at 95% | Pachysolen tannophilus | D-fructose + NADH + H+ | - |
? | |
| 1.1.1.9 | glycerol + NAD+ | poor substrate | Pachysolen tannophilus | ? | - |
? | |
| 1.1.1.9 | additional information | no oxidation of inositol, meso-erythritol and D-(+)-arabitol | Pachysolen tannophilus | ? | - |
? | |
| 1.1.1.9 | additional information | no oxidation of mannitol | Pachysolen tannophilus | ? | - |
? | |
| 1.1.1.9 | xylitol + NAD+ | - |
Pachysolen tannophilus | D-xylulose + NADH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | tetramer | 4 * 40000, SDS-PAGE | Pachysolen tannophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 55 | - |
- |
Pachysolen tannophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 35 | 68 | about half-maximal activity at 35°C and 68°C | Pachysolen tannophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 25 | - |
and below stable | Pachysolen tannophilus |
| 1.1.1.9 | 60 | - |
complete inactivation within 5 min | Pachysolen tannophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 9.1 | 10 | substrate oxidation, plateau | Pachysolen tannophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 8.7 | 10.5 | about 80% of maximal oxidation activity at pH 8.7 and 10.5 | Pachysolen tannophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 7 | 9 | stable | Pachysolen tannophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | additional information | no oxidation of ribitol or sorbitol with NADP+ | Pachysolen tannophilus | |
| 1.1.1.9 | NAD+ | - |
Pachysolen tannophilus | |
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