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Literature summary extracted from

  • Jakoby, W.B.; Fredericks, J.
    Erythritol dehydrogenase from Aerobacter aerogenes (1961), Biochim. Biophys. Acta, 48, 26-32.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.9 2-mercaptoethanol stabilizes during purification Klebsiella aerogenes
1.1.1.9 EDTA stabilizes during purification Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.9 EDTA
-
 Klebsiella aerogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.9 cytoplasm
-
 Klebsiella aerogenes 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.9 Mg2+ activation at high concentration Klebsiella aerogenes
1.1.1.9 Mn2+ requirement Klebsiella aerogenes
1.1.1.9 additional information no activation by Zn2+, Cu2+, Ca2+, Ni2+, Co2+, Fe2+ Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9 partial Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.9 3.1
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.9 2°C, 90% of maximal activity retained at pH 6.8 and 7.4 after 24 h Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-arabitol + NAD+
-
 Klebsiella aerogenes ?
-
 ?
1.1.1.9 D-xylulose + NADH + H+
-
 Klebsiella aerogenes xylitol + NAD+
-
 r
1.1.1.9 dihydroxyacetone + NADH
-
 Klebsiella aerogenes glycerol + NAD+
-
 ?
1.1.1.9 erythritol + NAD+
-
 Klebsiella aerogenes L-erythrulose + NADH
-
 r
1.1.1.9 glycerol + NAD+
-
 Klebsiella aerogenes ?
-
 ?
1.1.1.9 L-arabitol + NAD+
-
 Klebsiella aerogenes ?
-
 ?
1.1.1.9 L-erythrulose + NADH
-
 Klebsiella aerogenes erythritol + NAD+
-
 r
1.1.1.9 L-xylulose + NADH
-
 Klebsiella aerogenes L-xylitol + NAD+
-
 ?
1.1.1.9 additional information no oxidation of threitol, xylitol, sorbitol, D-iditol Klebsiella aerogenes ?
-
 ?
1.1.1.9 additional information no reduction of D-fructose, L-sorbose and D-tagatose Klebsiella aerogenes ?
-
 ?
1.1.1.9 additional information specific for polyols of 5 or less carbon bearing cis-hydroxy groups in C2 and C3 Klebsiella aerogenes ?
-
 ?
1.1.1.9 xylitol + NAD+
-
 Klebsiella aerogenes D-xylulose + NADH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 6.7
-
 reduction of erythrulose Klebsiella aerogenes
1.1.1.9 8.6
-
 oxidation of erythritol Klebsiella aerogenes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.9 6.8 7.4 90% of maximal activity retained at pH 6.8 and 7.4 at 2°C Klebsiella aerogenes
1.1.1.9 7
-
 24 h stable at 2°C Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 additional information no activity with NADPH Klebsiella aerogenes
1.1.1.9 additional information no activity with NADP+ Klebsiella aerogenes
1.1.1.9 additional information at high concentrations of xylitol 1.5% as effective as NAD+ Klebsiella aerogenes
1.1.1.9 NAD+
-
 Klebsiella aerogenes
1.1.1.9 NADH
-
 Klebsiella aerogenes