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Literature summary extracted from
- A sensitive and stereospecific enzymatic assay for xylulose (1959), J. Biol. Chem., 234, 758-761.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | cysteine | of partially purified preparation | Cavia porcellus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.9 | crude water extract from liver acetone-powder unstable | Cavia porcellus |
| 1.1.1.9 | freezing/thawing inactivates, 8 cycles lead to 89% loss of activity and 11 cycles to complete inactivation | Cavia porcellus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | D-xylulose | high concentration | Cavia porcellus | |
| 1.1.1.9 | additional information | no inhibition by EDTA or iodoacetate | Cavia porcellus | |
| 1.1.1.9 | PCMB | complete inhibition | Cavia porcellus | |
| 1.1.1.10 | iodoacetate | not inhibitory | Cavia porcellus | |
| 1.1.1.10 | additional information | not: EDTA | Cavia porcellus | |
| 1.1.1.10 | p-chloromercuribenzoate | - |
Cavia porcellus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.9 | 0.66 | - |
D-xylulose | - |
Cavia porcellus | |
| 1.1.1.10 | 0.29 | - |
L-xylulose | - |
Cavia porcellus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.9 | cytoplasm | - |
Cavia porcellus | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | additional information | no evidence for metal requirement | Cavia porcellus |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.1.1.9 | Acetone | stable to precipitation with 50% v/v | Cavia porcellus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.9 | Cavia porcellus | - |
- |
- |
| 1.1.1.10 | Cavia porcellus | - |
guinea pig | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.9 | partial | Cavia porcellus |
| 1.1.1.10 | partial | Cavia porcellus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.9 | liver | - |
Cavia porcellus | - |
| 1.1.1.10 | liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | additional information | - |
- |
Cavia porcellus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.9 | -10°C, acetone precipitate stable for weeks | Cavia porcellus |
| 1.1.1.9 | 0°C, partially purified stable for weeks | Cavia porcellus |
| 1.1.1.10 | frozen, ammonium sulfate precipitate, several days | Cavia porcellus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.9 | D-ribitol + NAD+ | - |
Cavia porcellus | D-ribulose + NADH | - |
? | |
| 1.1.1.9 | D-sorbitol + NAD+ | - |
Cavia porcellus | D-fructose + NADH + H+ | - |
? | |
| 1.1.1.9 | D-xylulose + NADH + H+ | - |
Cavia porcellus | xylitol + NAD+ | - |
r | |
| 1.1.1.9 | L-arabitol + NAD+ | - |
Cavia porcellus | ? | - |
? | |
| 1.1.1.9 | L-erythrulose + NADH | reduction at the same rate as D-xylulose | Cavia porcellus | erythritol + NAD+ | - |
? | |
| 1.1.1.9 | L-iditol + NAD+ | - |
Cavia porcellus | ? | - |
? | |
| 1.1.1.9 | additional information | no reduction of D-ribose and D-galacturonic acid | Cavia porcellus | ? | - |
? | |
| 1.1.1.9 | additional information | no oxidation of threitol, xylitol, sorbitol, D-iditol | Cavia porcellus | ? | - |
? | |
| 1.1.1.9 | additional information | no reduction of D-ribulose | Cavia porcellus | ? | - |
? | |
| 1.1.1.9 | additional information | no reduction of D/L-xylose | Cavia porcellus | ? | - |
? | |
| 1.1.1.9 | xylitol + NAD+ | - |
Cavia porcellus | D-xylulose + NADH + H+ | - |
r | |
| 1.1.1.10 | xylitol + NADP+ | - |
Cavia porcellus | L-xylulose + NADPH + H+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 7 | - |
substrate reduction | Cavia porcellus |
| 1.1.1.10 | 7 | - |
- |
Cavia porcellus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.9 | 4 | 8 | about 75% of maximal reduction activity at pH 4.0 and 8.0 | Cavia porcellus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.9 | additional information | no activity with NADPH | Cavia porcellus | |
| 1.1.1.9 | additional information | no activity with NADP+ | Cavia porcellus | |
| 1.1.1.9 | additional information | at high concentrations of xylitol 1.5% as effective as NAD+ | Cavia porcellus | |
| 1.1.1.9 | NAD+ | - |
Cavia porcellus | |
| 1.1.1.9 | NADH | - |
Cavia porcellus | |
| 1.1.1.10 | additional information | - |
Cavia porcellus |
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Release 2023.1 (January 2023)
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