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Literature summary extracted from

  • Carballo, J.; Martin, R.; Bernardo, A.; Gonzalez, J.
    Purification, characterization and some properties of diacetyl(acetoin) reductase from Enterobacter aerogenes (1991), Eur. J. Biochem., 198, 327-332.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.4 0.16
-
 NAD+
-
 Klebsiella aerogenes
1.1.1.4 0.4
-
 acetoin
-
 Klebsiella aerogenes
1.1.1.4 1.6
-
 diacetyl
-
 Klebsiella aerogenes
1.1.1.4 2.6
-
 2,3-Butanediol
-
 Klebsiella aerogenes
1.1.1.4 6
-
 2,3-pentanediol
-
 Klebsiella aerogenes
1.1.1.4 18
-
 Methyl pyruvate
-
 Klebsiella aerogenes
1.1.1.4 20
-
 ethyl pyruvate
-
 Klebsiella aerogenes
1.1.1.4 75
-
 methyl glyoxal
-
 Klebsiella aerogenes
1.1.1.304 0.005
-
 NADH cosubstrate acetoin, pH 7.0, 25°C Klebsiella aerogenes
1.1.1.304 0.007
-
 NADH cosubstrate diacetyl, pH 7.0, 25°C Klebsiella aerogenes
1.1.1.304 1.6
-
 diacetyl pH 7.0, 25°C Klebsiella aerogenes
1.1.1.304 6
-
 pentane-2,3-dione pH 7.0, 25°C Klebsiella aerogenes
1.1.1.304 18
-
 Methyl pyruvate pH 7.0, 25°C Klebsiella aerogenes
1.1.1.304 20
-
 ethyl pyruvate pH 7.0, 25°C Klebsiella aerogenes
1.1.1.304 75
-
 methyl glyoxal pH 7.0, 25°C Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 28000
-
 2 * 28000, SDS-PAGE Klebsiella aerogenes
1.1.1.4 61000
-
 gel filtration Klebsiella aerogenes
1.1.1.304 28000
-
 2 * 28000, SDS-PAGE Klebsiella aerogenes
1.1.1.304 61000
-
 gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Klebsiella aerogenes
-
 authors suggest systematic name: -glycol:NAD+ oxidoreductase ???
-
1.1.1.304 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.4
-
 Klebsiella aerogenes
1.1.1.304
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.4 270
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 2,3-butanediol + NAD+
-
 Klebsiella aerogenes acetoin + NADH
-
 r
1.1.1.4 2,3-pentanediol + NAD+
-
 Klebsiella aerogenes ?
-
 r
1.1.1.4 acetoin + NADH + H+
-
 Klebsiella aerogenes 2,3-butanediol + NAD+
-
 r
1.1.1.4 diacetyl + NADH
-
 Klebsiella aerogenes 2,3-butanediol + NAD+
-
 ?
1.1.1.4 ethyl pyruvate + NADH
-
 Klebsiella aerogenes ?
-
 ?
1.1.1.4 methyl glyoxal + NADH
-
 Klebsiella aerogenes ?
-
 ?
1.1.1.4 methyl pyruvate + NADH
-
 Klebsiella aerogenes ?
-
 ?
1.1.1.304 2,3-pentanedione + NADH + H+ 85.6% of the activity with diacetyl Klebsiella aerogenes 3-hydroxy-2-pentanone + NAD+
-
 ?
1.1.1.304 diacetyl + NADH + H+
-
 Klebsiella aerogenes (S)-acetoin + NAD+
-
 ?
1.1.1.304 ethyl pyruvate + NADH + H+ 57.7% of the activity with diacetyl Klebsiella aerogenes ? + NAD+
-
 ?
1.1.1.304 methyl glyoxal + NADH + H+ 11% of the activity with diacetyl Klebsiella aerogenes ? + NAD+
-
 ?
1.1.1.304 methyl pyruvate + NADH + H+ 49% of the activity with diacetyl Klebsiella aerogenes ? + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.4 dimer 2 * 28000, SDS-PAGE Klebsiella aerogenes
1.1.1.304 dimer 2 * 28000, SDS-PAGE Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.4 10
-
-
 Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+ no activity with NADP+ as cofactor Klebsiella aerogenes
1.1.1.304 NADH specific for beta-NADH Klebsiella aerogenes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.304 Klebsiella aerogenes isoelectric focusing
-
 6.8