BRENDA - Enzyme Database show

Characterization of yeast homoserine dehydrogenase, an antifungal target: the invariant histidine 309 is important for enzyme integrity

Jacques, S.L.; Nieman, C.; Bareich, D.; Broadhead, G.; Kinach, R.; Honek, J.F.; Wright, G.D.; Biochim. Biophys. Acta 1544, 28-41 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.3
overexpression in Escherichia coli
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.3
H309A
decrease of catalytic activity and elimination of substrate inhibition
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Saccharomyces cerevisiae
P31116
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
overexpressed in Escherichia coli
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.3
23.7
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246402
Saccharomyces cerevisiae
L-homoserine + NAD(P)+
-
246402
Saccharomyces cerevisiae
-
1.1.1.3
L-homoserine + NAD(P)+
-
246402
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + NAD(P)H
-
246402
Saccharomyces cerevisiae
r
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
overexpression in Escherichia coli
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.3
H309A
decrease of catalytic activity and elimination of substrate inhibition
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
overexpressed in Escherichia coli
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.3
23.7
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246402
Saccharomyces cerevisiae
L-homoserine + NAD(P)+
-
246402
Saccharomyces cerevisiae
-
1.1.1.3
L-homoserine + NAD(P)+
-
246402
Saccharomyces cerevisiae
L-aspartate 4-semialdehyde + NAD(P)H
-
246402
Saccharomyces cerevisiae
r