BRENDA - Enzyme Database show

Purification and characterization of homoserine dehydrogenase from spinach leaves

Pavagi, S.; Kochhar, S.; Kochhar, V.K.; Sane, P.V.; Biochem. Mol. Biol. Int. 36, 649-658 (1995)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.3
Calmodulin
in the presence of above 0.1 mM Ca2+
Spinacia oleracea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
aspartate
-
Spinacia oleracea
1.1.1.3
EGTA
-
Spinacia oleracea
1.1.1.3
L-cysteine
-
Spinacia oleracea
1.1.1.3
L-threonine
-
Spinacia oleracea
1.1.1.3
lysine
-
Spinacia oleracea
1.1.1.3
methionine
-
Spinacia oleracea
1.1.1.3
Trifluoperazine
-
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.1
-
DL-aspartate 4-semialdehyde
-
Spinacia oleracea
1.1.1.3
0.3
-
NADH
-
Spinacia oleracea
1.1.1.3
0.5
-
L-aspartate 4-semialdehyde
-
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
55000
-
4 * 55000, SDS-PAGE
Spinacia oleracea
1.1.1.3
220000
-
gel filtration
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Spinacia oleracea
-
also shows aspartase kinase activity; spinach
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
-
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246395
Spinacia oleracea
L-homoserine + NAD(P)+
-
246395
Spinacia oleracea
r
1.1.1.3
L-homoserine + NAD(P)+
-
246395
Spinacia oleracea
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.3
tetramer
4 * 55000, SDS-PAGE
Spinacia oleracea
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.3
Calmodulin
in the presence of above 0.1 mM Ca2+
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
aspartate
-
Spinacia oleracea
1.1.1.3
EGTA
-
Spinacia oleracea
1.1.1.3
L-cysteine
-
Spinacia oleracea
1.1.1.3
L-threonine
-
Spinacia oleracea
1.1.1.3
lysine
-
Spinacia oleracea
1.1.1.3
methionine
-
Spinacia oleracea
1.1.1.3
Trifluoperazine
-
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.1
-
DL-aspartate 4-semialdehyde
-
Spinacia oleracea
1.1.1.3
0.3
-
NADH
-
Spinacia oleracea
1.1.1.3
0.5
-
L-aspartate 4-semialdehyde
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
55000
-
4 * 55000, SDS-PAGE
Spinacia oleracea
1.1.1.3
220000
-
gel filtration
Spinacia oleracea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
-
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246395
Spinacia oleracea
L-homoserine + NAD(P)+
-
246395
Spinacia oleracea
r
1.1.1.3
L-homoserine + NAD(P)+
-
246395
Spinacia oleracea
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.3
tetramer
4 * 55000, SDS-PAGE
Spinacia oleracea