BRENDA - Enzyme Database show

Engineering the homoserine dehydrogenase and threonine dehydratase control points to analyse flux towards L-isoleucine in Corynebacterium glutamicum

Morbach, S.; Kelle, R.; Winkels, S.; Sahm, H.; Eggeling, L.; Appl. Microbiol. Biotechnol. 45, 612-620 (1996)
No PubMed abstract available

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.3
G378E
feedback resistance of the enzyme
Corynebacterium glutamicum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-cysteine
-
Corynebacterium glutamicum
1.1.1.3
L-threonine
-
Corynebacterium glutamicum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Corynebacterium glutamicum
-
-
-
4.3.1.19
Corynebacterium glutamicum
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.3
additional information
-
-
Corynebacterium glutamicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-homoserine + NAD(P)+
-
246394
Corynebacterium glutamicum
L-aspartate 4-semialdehyde + NAD(P)H
-
246394
Corynebacterium glutamicum
-
4.3.1.19
L-threonine
-
246394
Corynebacterium glutamicum
2-oxobutanoate + NH3
-
246394
Corynebacterium glutamicum
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.3
G378E
feedback resistance of the enzyme
Corynebacterium glutamicum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-cysteine
-
Corynebacterium glutamicum
1.1.1.3
L-threonine
-
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.3
additional information
-
-
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-homoserine + NAD(P)+
-
246394
Corynebacterium glutamicum
L-aspartate 4-semialdehyde + NAD(P)H
-
246394
Corynebacterium glutamicum
-
4.3.1.19
L-threonine
-
246394
Corynebacterium glutamicum
2-oxobutanoate + NH3
-
246394
Corynebacterium glutamicum
?