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Studies on homoserine dehydrogenase from an extreme thermophile, Thermus flavus AT-62. Partial purification and properties

Saiki, T.; Shinshi, H.; Arima, K.; J. Biochem. 74, 1239-1248 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-threonine
not inhibitory
Thermus thermophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.17
0.18
NADP+
-
Thermus thermophilus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.3
K+
activation
Thermus thermophilus
1.1.1.3
Na+
activation
Thermus thermophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Thermus thermophilus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
partial
Thermus thermophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246393
Thermus thermophilus
L-homoserine + NAD(P)+
-
246393
Thermus thermophilus
r
1.1.1.3
L-homoserine + NAD(P)+
-
246393
Thermus thermophilus
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.3
additional information
-
above 50C temperature dependent conformational change
Thermus thermophilus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.3
70
-
up to
Thermus thermophilus
1.1.1.3
80
-
slow inactivation, protection by K+, Na+
Thermus thermophilus
1.1.1.3
90
-
rapid inactivation
Thermus thermophilus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.3
9
-
-
Thermus thermophilus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-threonine
not inhibitory
Thermus thermophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.17
0.18
NADP+
-
Thermus thermophilus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.3
K+
activation
Thermus thermophilus
1.1.1.3
Na+
activation
Thermus thermophilus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
partial
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246393
Thermus thermophilus
L-homoserine + NAD(P)+
-
246393
Thermus thermophilus
r
1.1.1.3
L-homoserine + NAD(P)+
-
246393
Thermus thermophilus
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.3
additional information
-
above 50C temperature dependent conformational change
Thermus thermophilus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.3
70
-
up to
Thermus thermophilus
1.1.1.3
80
-
slow inactivation, protection by K+, Na+
Thermus thermophilus
1.1.1.3
90
-
rapid inactivation
Thermus thermophilus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.3
9
-
-
Thermus thermophilus