BRENDA - Enzyme Database show

Threonine-sensitive aspartate kinase and homoserine dehydrogenase from Pisum sativum

Aarnes, H.; Rognes, S.E.; Phytochemistry 13, 2717-2724 (1974)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-cysteine
-
Pisum sativum
1.1.1.3
L-threonine
sensitive and insensitive isozymes
Pisum sativum
1.1.1.3
methionine
-
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.08
-
L-aspartate 4-semialdehyde
threonine insensitive isozyme
Pisum sativum
1.1.1.3
0.5
-
L-aspartate 4-semialdehyde
threonine sensitive isozyme
Pisum sativum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Pisum sativum
-
pea
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
-
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
seedling
-
Pisum sativum
-
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.3
-25°C, 50 mM potassium phosphate buffer, pH 7.2, 15% v/v glycerol, 1 mM EDTA, 1 mM threonine, 14 mM 2-mercaptoethanol
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246391
Pisum sativum
L-homoserine + NAD(P)+
-
246391
Pisum sativum
r
1.1.1.3
L-homoserine + NAD(P)+
-
246391
Pisum sativum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADP+
-
Pisum sativum
1.1.1.3
NADPH
-
Pisum sativum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADP+
-
Pisum sativum
1.1.1.3
NADPH
-
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-cysteine
-
Pisum sativum
1.1.1.3
L-threonine
sensitive and insensitive isozymes
Pisum sativum
1.1.1.3
methionine
-
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.08
-
L-aspartate 4-semialdehyde
threonine insensitive isozyme
Pisum sativum
1.1.1.3
0.5
-
L-aspartate 4-semialdehyde
threonine sensitive isozyme
Pisum sativum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
-
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
seedling
-
Pisum sativum
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.3
-25°C, 50 mM potassium phosphate buffer, pH 7.2, 15% v/v glycerol, 1 mM EDTA, 1 mM threonine, 14 mM 2-mercaptoethanol
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246391
Pisum sativum
L-homoserine + NAD(P)+
-
246391
Pisum sativum
r
1.1.1.3
L-homoserine + NAD(P)+
-
246391
Pisum sativum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r