BRENDA - Enzyme Database show

Comparison of homoserine dehydrogenase from different plant sources

Grego, S.; Tricoli, D.; Di Marco, G.; Phytochemistry 19, 1619-1623 (1980)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-threonine
degree of inhibition depends on age of plant; sensitive and insensitive isozymes
Pisum sativum
1.1.1.3
L-threonine
degree of inhibition depends on age of plant; sensitive and insensitive isozymes
Ricinus communis
1.1.1.3
L-threonine
degree of inhibition depends on age of plant; sensitive and insensitive isozymes
Triticum aestivum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.031
-
NADPH
threonine sensitive isozyme
Triticum aestivum
1.1.1.3
0.032
0.036
NADPH
threonine resistant isozyme
Triticum aestivum
1.1.1.3
0.043
-
NADPH
threonine sensitive isozyme
Triticum aestivum
1.1.1.3
0.13
0.15
L-aspartate 4-semialdehyde
threonine resistant isozyme
Triticum aestivum
1.1.1.3
0.24
0.25
L-aspartate 4-semialdehyde
threonine sensitive isozyme
Triticum aestivum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.3
Cs+
activation of threonine sensitive enzyme
Pisum sativum
1.1.1.3
Cs+
activation of threonine sensitive enzyme
Ricinus communis
1.1.1.3
Cs+
activation of threonine sensitive enzyme
Triticum aestivum
1.1.1.3
K+
activation of threonine sensitive isozyme
Pisum sativum
1.1.1.3
K+
activation of threonine sensitive isozyme
Ricinus communis
1.1.1.3
K+
activation of threonine sensitive isozyme
Triticum aestivum
1.1.1.3
Li+
activation of threonine sensitive isozyme
Pisum sativum
1.1.1.3
Li+
activation of threonine sensitive isozyme
Ricinus communis
1.1.1.3
Li+
activation of threonine sensitive isozyme
Triticum aestivum
1.1.1.3
Na+
activation of threonine sensitive form
Pisum sativum
1.1.1.3
Na+
activation of threonine sensitive form
Ricinus communis
1.1.1.3
Na+
activation of threonine sensitive form
Triticum aestivum
1.1.1.3
NH4+
activation of threonine sensitive isozyme
Pisum sativum
1.1.1.3
NH4+
activation of threonine sensitive isozyme
Ricinus communis
1.1.1.3
NH4+
activation of threonine sensitive isozyme
Triticum aestivum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
75000
-
gel filtration, threonine insensitive isozyme
Pisum sativum
1.1.1.3
75000
-
gel filtration, threonine insensitive isozyme
Triticum aestivum
1.1.1.3
80000
-
disc gel electrophoresis, threonine insensitive isozyme
Ricinus communis
1.1.1.3
280000
-
disc gel electrophoresis, threonine sensitive isozyme
Ricinus communis
1.1.1.3
290000
-
gel filtration, threonine sensitive isozyme
Triticum aestivum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Triticum aestivum
-
L-homoserine + NAD(P)+
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Pisum sativum
-
L-homoserine + NAD(P)+
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Ricinus communis
-
L-homoserine + NAD(P)+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Pisum sativum
-
pea
-
1.1.1.3
Ricinus communis
-
castor bean
-
1.1.1.3
Triticum aestivum
-
wheat
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
-
Ricinus communis
1.1.1.3
-
Triticum aestivum
1.1.1.3
-
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
seedling
-
Ricinus communis
-
1.1.1.3
seedling
-
Triticum aestivum
-
1.1.1.3
seedling
-
Pisum sativum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.3
0.17
-
-
Triticum aestivum
1.1.1.3
2.8
-
-
Pisum sativum
1.1.1.3
6.8
-
-
Ricinus communis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Triticum aestivum
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Pisum sativum
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Pisum sativum
L-homoserine + NAD(P)+
-
246388
Pisum sativum
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Ricinus communis
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Ricinus communis
L-homoserine + NAD(P)+
-
246388
Ricinus communis
r
1.1.1.3
L-homoserine + NAD(P)+
-
246388
Triticum aestivum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246388
Pisum sativum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246388
Ricinus communis
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Pisum sativum
1.1.1.3
NADH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Ricinus communis
1.1.1.3
NADH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Triticum aestivum
1.1.1.3
NADPH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Pisum sativum
1.1.1.3
NADPH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Ricinus communis
1.1.1.3
NADPH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Triticum aestivum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Pisum sativum
1.1.1.3
NADH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Ricinus communis
1.1.1.3
NADH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Triticum aestivum
1.1.1.3
NADPH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Pisum sativum
1.1.1.3
NADPH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Ricinus communis
1.1.1.3
NADPH
threonine sensitive isozyme can use NADPH or NADH, threonine insensitive isozyme can use NADPH only
Triticum aestivum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-threonine
degree of inhibition depends on age of plant; sensitive and insensitive isozymes
Pisum sativum
1.1.1.3
L-threonine
degree of inhibition depends on age of plant; sensitive and insensitive isozymes
Ricinus communis
1.1.1.3
L-threonine
degree of inhibition depends on age of plant; sensitive and insensitive isozymes
Triticum aestivum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.031
-
NADPH
threonine sensitive isozyme
Triticum aestivum
1.1.1.3
0.032
0.036
NADPH
threonine resistant isozyme
Triticum aestivum
1.1.1.3
0.043
-
NADPH
threonine sensitive isozyme
Triticum aestivum
1.1.1.3
0.13
0.15
L-aspartate 4-semialdehyde
threonine resistant isozyme
Triticum aestivum
1.1.1.3
0.24
0.25
L-aspartate 4-semialdehyde
threonine sensitive isozyme
Triticum aestivum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.3
Cs+
activation of threonine sensitive enzyme
Pisum sativum
1.1.1.3
Cs+
activation of threonine sensitive enzyme
Ricinus communis
1.1.1.3
Cs+
activation of threonine sensitive enzyme
Triticum aestivum
1.1.1.3
K+
activation of threonine sensitive isozyme
Pisum sativum
1.1.1.3
K+
activation of threonine sensitive isozyme
Ricinus communis
1.1.1.3
K+
activation of threonine sensitive isozyme
Triticum aestivum
1.1.1.3
Li+
activation of threonine sensitive isozyme
Pisum sativum
1.1.1.3
Li+
activation of threonine sensitive isozyme
Ricinus communis
1.1.1.3
Li+
activation of threonine sensitive isozyme
Triticum aestivum
1.1.1.3
Na+
activation of threonine sensitive form
Pisum sativum
1.1.1.3
Na+
activation of threonine sensitive form
Ricinus communis
1.1.1.3
Na+
activation of threonine sensitive form
Triticum aestivum
1.1.1.3
NH4+
activation of threonine sensitive isozyme
Pisum sativum
1.1.1.3
NH4+
activation of threonine sensitive isozyme
Ricinus communis
1.1.1.3
NH4+
activation of threonine sensitive isozyme
Triticum aestivum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
75000
-
gel filtration, threonine insensitive isozyme
Pisum sativum
1.1.1.3
75000
-
gel filtration, threonine insensitive isozyme
Triticum aestivum
1.1.1.3
80000
-
disc gel electrophoresis, threonine insensitive isozyme
Ricinus communis
1.1.1.3
280000
-
disc gel electrophoresis, threonine sensitive isozyme
Ricinus communis
1.1.1.3
290000
-
gel filtration, threonine sensitive isozyme
Triticum aestivum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Triticum aestivum
-
L-homoserine + NAD(P)+
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Pisum sativum
-
L-homoserine + NAD(P)+
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Ricinus communis
-
L-homoserine + NAD(P)+
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
-
Ricinus communis
1.1.1.3
-
Triticum aestivum
1.1.1.3
-
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
seedling
-
Ricinus communis
-
1.1.1.3
seedling
-
Triticum aestivum
-
1.1.1.3
seedling
-
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.3
0.17
-
-
Triticum aestivum
1.1.1.3
2.8
-
-
Pisum sativum
1.1.1.3
6.8
-
-
Ricinus communis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Triticum aestivum
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Pisum sativum
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Pisum sativum
L-homoserine + NAD(P)+
-
246388
Pisum sativum
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Ricinus communis
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246388
Ricinus communis
L-homoserine + NAD(P)+
-
246388
Ricinus communis
r
1.1.1.3
L-homoserine + NAD(P)+
-
246388
Triticum aestivum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246388
Pisum sativum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246388
Ricinus communis
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r