BRENDA - Enzyme Database show

Isolation and characterization of two homoserine dehydrogenases from maize suspension cultures

Walter, T.J.; Connelly, J.A.; Gengenbach, B.G.; Wold, F.; J. Biol. Chem. 254, 1349-1355 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-threonine
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.032
0.036
NADPH
threonine resistant isozyme
Zea mays
1.1.1.3
0.04
-
NADPH
threonine sensitive isozyme
Zea mays
1.1.1.3
0.13
0.15
L-aspartate 4-semialdehyde
threonine resistant isozyme
Zea mays
1.1.1.3
0.24
0.25
L-aspartate 4-semialdehyde
threonine sensitive isozyme
Zea mays
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
38000
-
2 * 38000, SDS-PAGE, threonine resistant isozyme
Zea mays
1.1.1.3
70000
-
gel filtration, threonine insensitive isozyme
Zea mays
1.1.1.3
89000
-
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays
1.1.1.3
93000
-
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays
1.1.1.3
190000
-
gel filtration threonine sensitive isozyme
Zea mays
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Zea mays
-
corn
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
threonine sensitive and threonine insensitive isozymes
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
cell suspension culture
-
Zea mays
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.3
343
-
-
Zea mays
1.1.1.3
360
-
-
Zea mays
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.3
-20°C, 30 mM potassium phosphate buffer, pH 7.5, 50% v/v glycerol, threonine resistant isozyme at least 2 months stable, threonine sensitive isozyme at least 4 months stable
Zea mays
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.3
dimer
2 * 38000, SDS-PAGE, threonine resistant isozyme; x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays
1.1.1.3
tetramer
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADPH
-
Zea mays
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADPH
-
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-threonine
-
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.032
0.036
NADPH
threonine resistant isozyme
Zea mays
1.1.1.3
0.04
-
NADPH
threonine sensitive isozyme
Zea mays
1.1.1.3
0.13
0.15
L-aspartate 4-semialdehyde
threonine resistant isozyme
Zea mays
1.1.1.3
0.24
0.25
L-aspartate 4-semialdehyde
threonine sensitive isozyme
Zea mays
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
38000
-
2 * 38000, SDS-PAGE, threonine resistant isozyme
Zea mays
1.1.1.3
70000
-
gel filtration, threonine insensitive isozyme
Zea mays
1.1.1.3
89000
-
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays
1.1.1.3
93000
-
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays
1.1.1.3
190000
-
gel filtration threonine sensitive isozyme
Zea mays
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
threonine sensitive and threonine insensitive isozymes
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
cell suspension culture
-
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.3
343
-
-
Zea mays
1.1.1.3
360
-
-
Zea mays
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.3
-20°C, 30 mM potassium phosphate buffer, pH 7.5, 50% v/v glycerol, threonine resistant isozyme at least 2 months stable, threonine sensitive isozyme at least 4 months stable
Zea mays
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.3
dimer
2 * 38000, SDS-PAGE, threonine resistant isozyme; x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays
1.1.1.3
tetramer
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme
Zea mays