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Literature summary extracted from

  • Walter, T.J.; Connelly, J.A.; Gengenbach, B.G.; Wold, F.
    Isolation and characterization of two homoserine dehydrogenases from maize suspension cultures (1979), J. Biol. Chem., 254, 1349-1355.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.3 L-threonine
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.3 0.036
-
 NADPH threonine resistant isozyme Zea mays
1.1.1.3 0.036
-
 NADPH threonine-resistant enzyme, pH and temperature not specified in the publication Zea mays
1.1.1.3 0.04
-
 NADPH threonine sensitive isozyme Zea mays
1.1.1.3 0.04
-
 NADPH threonine-sensitive enzyme, pH and temperature not specified in the publication Zea mays
1.1.1.3 0.15
-
 L-aspartate 4-semialdehyde threonine resistant isozyme Zea mays
1.1.1.3 0.15
-
 L-aspartate 4-semialdehyde threonine-resistant enzyme, pH and temperature not specified in the publication Zea mays
1.1.1.3 0.25
-
 L-aspartate 4-semialdehyde threonine sensitive isozyme Zea mays
1.1.1.3 0.25
-
 L-aspartate 4-semialdehyde threonine-sensitive enzyme, pH and temperature not specified in the publication Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.3 38000
-
 2 * 38000, SDS-PAGE, threonine resistant isozyme Zea mays
1.1.1.3 70000
-
 gel filtration, threonine insensitive isozyme Zea mays
1.1.1.3 89000
-
 x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme Zea mays
1.1.1.3 93000
-
 x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme Zea mays
1.1.1.3 190000
-
 gel filtration threonine sensitive isozyme Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Zea mays
-
 corn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.3 threonine sensitive and threonine insensitive isozymes Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.3 cell suspension culture
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.3 343
-
-
 Zea mays
1.1.1.3 360
-
-
 Zea mays

Storage Stability

EC Number Storage Stability Organism
1.1.1.3 -20°C, 30 mM potassium phosphate buffer, pH 7.5, 50% v/v glycerol, threonine resistant isozyme at least 2 months stable, threonine sensitive isozyme at least 4 months stable Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 L-aspartate 4-semialdehyde + NADPH + H+
-
 Zea mays L-homoserine + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.3 dimer 2 * 38000, SDS-PAGE, threonine resistant isozyme Zea mays
1.1.1.3 dimer x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme Zea mays
1.1.1.3 tetramer x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.3 NADPH
-
 Zea mays