BRENDA - Enzyme Database show

Localisation and characterisation of homoserine dehydrogenase isolated from barley and pea leaves

Sainis, J.K.; Mayne, R.G.; Wallsgrove, R.M.; Lea, P.J.; Miflin, B.J.; Planta 152, 491-496 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-cysteine
slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme
Hordeum vulgare
1.1.1.3
L-cysteine
slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme
Pisum sativum
1.1.1.3
L-threonine
-
Hordeum vulgare
1.1.1.3
L-threonine
-
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.027
-
NADPH
isozyme II
Pisum sativum
1.1.1.3
0.04
-
aspartate 4-semialdehyde
isozyme II
Pisum sativum
1.1.1.3
0.046
-
NADPH
isozyme I
Pisum sativum
1.1.1.3
0.066
-
L-aspartate 4-semialdehyde
isozyme I
Pisum sativum
1.1.1.3
0.1
-
L-aspartate 4-semialdehyde
isozyme I
Hordeum vulgare
1.1.1.3
0.36
0.4
L-aspartate 4-semialdehyde
isozyme II
Hordeum vulgare
1.1.1.3
0.46
-
NADH
isozyme II
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.3
chloroplast
isozyme I
Hordeum vulgare
9507
-
1.1.1.3
chloroplast
isozyme I
Pisum sativum
9507
-
1.1.1.3
cytoplasm
isozyme II
Hordeum vulgare
5737
-
1.1.1.3
cytoplasm
isozyme II
Pisum sativum
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
34000
-
gel filtration, isozyme II, cytoplasm
Pisum sativum
1.1.1.3
69000
-
gel filtration, isozyme II, cytoplasm
Hordeum vulgare
1.1.1.3
168000
174000
gel filtration, isozyme I, chloroplasts
Hordeum vulgare
1.1.1.3
168000
174000
gel filtration, isozyme I, chloroplasts
Pisum sativum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Hordeum vulgare
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
L-homoserine + NAD(P)+
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Pisum sativum
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
L-homoserine + NAD(P)+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Hordeum vulgare
-
barley
-
1.1.1.3
Pisum sativum
-
pea
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
separation of the two isozymes using Blue-Sepharose chromatography
Pisum sativum
1.1.1.3
separation of the two isozymes using Matrex Gel Red A affinity chromatography
Hordeum vulgare
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
leaf
-
Hordeum vulgare
-
1.1.1.3
leaf
-
Pisum sativum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246382
Hordeum vulgare
L-homoserine + NAD(P)+
-
246382
Hordeum vulgare
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246382
Pisum sativum
L-homoserine + NAD(P)+
-
246382
Pisum sativum
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
246382
Hordeum vulgare
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
246382
Pisum sativum
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246382
Hordeum vulgare
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246382
Pisum sativum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADH
-
Hordeum vulgare
1.1.1.3
NADH
-
Pisum sativum
1.1.1.3
NADPH
preferred
Hordeum vulgare
1.1.1.3
NADPH
preferred
Pisum sativum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.3
NADH
-
Hordeum vulgare
1.1.1.3
NADH
-
Pisum sativum
1.1.1.3
NADPH
preferred
Hordeum vulgare
1.1.1.3
NADPH
preferred
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
L-cysteine
slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme
Hordeum vulgare
1.1.1.3
L-cysteine
slight inhibition of chloroplast isozyme, strong inhibition of cytoplasmic isozyme
Pisum sativum
1.1.1.3
L-threonine
-
Hordeum vulgare
1.1.1.3
L-threonine
-
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.3
0.027
-
NADPH
isozyme II
Pisum sativum
1.1.1.3
0.04
-
aspartate 4-semialdehyde
isozyme II
Pisum sativum
1.1.1.3
0.046
-
NADPH
isozyme I
Pisum sativum
1.1.1.3
0.066
-
L-aspartate 4-semialdehyde
isozyme I
Pisum sativum
1.1.1.3
0.1
-
L-aspartate 4-semialdehyde
isozyme I
Hordeum vulgare
1.1.1.3
0.36
0.4
L-aspartate 4-semialdehyde
isozyme II
Hordeum vulgare
1.1.1.3
0.46
-
NADH
isozyme II
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.3
chloroplast
isozyme I
Hordeum vulgare
9507
-
1.1.1.3
chloroplast
isozyme I
Pisum sativum
9507
-
1.1.1.3
cytoplasm
isozyme II
Hordeum vulgare
5737
-
1.1.1.3
cytoplasm
isozyme II
Pisum sativum
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.3
34000
-
gel filtration, isozyme II, cytoplasm
Pisum sativum
1.1.1.3
69000
-
gel filtration, isozyme II, cytoplasm
Hordeum vulgare
1.1.1.3
168000
174000
gel filtration, isozyme I, chloroplasts
Hordeum vulgare
1.1.1.3
168000
174000
gel filtration, isozyme I, chloroplasts
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Hordeum vulgare
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
L-homoserine + NAD(P)+
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
Pisum sativum
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
L-homoserine + NAD(P)+
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
separation of the two isozymes using Blue-Sepharose chromatography
Pisum sativum
1.1.1.3
separation of the two isozymes using Matrex Gel Red A affinity chromatography
Hordeum vulgare
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.3
leaf
-
Hordeum vulgare
-
1.1.1.3
leaf
-
Pisum sativum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246382
Hordeum vulgare
L-homoserine + NAD(P)+
-
246382
Hordeum vulgare
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
-
246382
Pisum sativum
L-homoserine + NAD(P)+
-
246382
Pisum sativum
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
246382
Hordeum vulgare
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-aspartate 4-semialdehyde + NAD(P)H
third reaction in the pathway between aspartate and the amino acids threonine, isoleucine, methionine
246382
Pisum sativum
L-homoserine + NAD(P)+
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246382
Hordeum vulgare
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r
1.1.1.3
L-homoserine + NAD(P)+
-
246382
Pisum sativum
L-aspartate 4-semialdehyde + NAD(P)H
-
-
-
r