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Literature summary extracted from

  • Takamura, Y.; Takamura, T.; Soejima, M.; Uemura, T.
    Studies on the induced synthesis of maleate cis-trans isomerase by malonate. Part III. Purification and properties of maleate cis-trans isomerase induced by malonate (1969), Agric. Biol. Chem., 33, 718-728.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.2.1.1 Cu2+
-
 Alcaligenes faecalis
5.2.1.1 cyanide KCN, 1 mM, 20% inhibition Alcaligenes faecalis
5.2.1.1 EDTA 1 mM, 7% inhibition Alcaligenes faecalis
5.2.1.1 Hg2+
-
 Alcaligenes faecalis
5.2.1.1 iodoacetate 1 mM 73% inhibition Alcaligenes faecalis
5.2.1.1 PCMB 0.1 mM, complete inhibition; inhibition is overcome by the addition of sulfhydryl compounds Alcaligenes faecalis
5.2.1.1 Sodium periodate 1 mM, complete inhibition Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2.1.1 2.8
-
 Maleate
-
 Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.1 Alcaligenes faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.2.1.1
-
 Alcaligenes faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.2.1.1 additional information
-
-
 Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.1 Maleate reverse reaction is not observed Alcaligenes faecalis Fumarate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.2.1.1 30
-
 15 min, stable below Alcaligenes faecalis
5.2.1.1 40
-
 15 min, about 25% loss of activity Alcaligenes faecalis
5.2.1.1 50
-
 15 min, complete loss of activity Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.1 8.3
-
-
 Alcaligenes faecalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.2.1.1 7 9.3 pH 7: about 60% of maximal activity, pH 9.3: about 40% of maximal activity Alcaligenes faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.2.1.1 5 7 stable over this range in presence of dithiothreitol for a few weeks. In absence of dithiothreitol the activity is markedly decreased, especially in the alkaline region Alcaligenes faecalis
5.2.1.1 6
-
 most stable in acetate buffer at this pH Alcaligenes faecalis