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Literature summary extracted from

  • Seltzer, S.
    cis-trans Isomerization (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 6, 381-406.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.2.1.1 mercaptans
-
 Pseudomonas fluorescens
5.2.1.1 mercaptans required for activity Pseudomonas sp.
5.2.1.2 GSH required as coenzyme Vibrio sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.2.1.1
-
 Pseudomonas sp.

General Stability

EC Number General Stability Organism
5.2.1.1 crystallization can be accompanied by a 50% loss in activity Pseudomonas sp.
5.2.1.2 10-15 mM mercaptoethanol stabilizes Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.2.1.1 Bromoacetate
-
 Pseudomonas fluorescens
5.2.1.1 Bromoacetate
-
 Pseudomonas sp.
5.2.1.1 cyanide 1 mM, weak inhibition Alcaligenes faecalis
5.2.1.1 deoxycholate
-
 Pseudomonas sp.
5.2.1.1 EDTA
-
 Alcaligenes faecalis
5.2.1.1 EDTA 0.1 mM, 20% inhibition Pseudomonas sp.
5.2.1.1 Fe2+
-
 Alcaligenes faecalis
5.2.1.1 Hg2+
-
 Alcaligenes faecalis
5.2.1.1 iodoacetate
-
 Alcaligenes faecalis
5.2.1.1 iodoacetate 0.01 mM, complete inhibition Pseudomonas sp.
5.2.1.1 N-bromosuccinimide 1 mM, weak inhibition Alcaligenes faecalis
5.2.1.1 NaCN 0.6 mM, 20% inhibition Pseudomonas sp.
5.2.1.1 NaF 0.6-3 mM, 18-45% inhibition Pseudomonas sp.
5.2.1.1 p-Substituted mercuribenzoate
-
 Alcaligenes faecalis
5.2.1.1 p-Substituted mercuribenzoate 0.01 mM, complete inhibition Pseudomonas sp.
5.2.1.1 Semicarbazide 1 mM, weak inhibition Alcaligenes faecalis
5.2.1.1 Sodium periodate
-
 Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2.1.1 0.3
-
 Maleate
-
 Pseudomonas sp.
5.2.1.1 2.8
-
 Maleate
-
 Alcaligenes faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.2.1.1 CO2 increases activity by 20-30% Alcaligenes faecalis
5.2.1.1 Fe2+ increases activity by 20-30% Alcaligenes faecalis
5.2.1.1 Mn2+ increases activity by 20-30% Alcaligenes faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.2.1.1 74000
-
 sedimentation velocity experiments Pseudomonas fluorescens
5.2.1.1 74000
-
 sedimentation velocity experiments Pseudomonas sp.
5.2.1.1 100000
-
 gel filtration, sedimentation velocity studies Alcaligenes faecalis
5.3.3.7 78000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.2.1.1 additional information Alcaligenes faecalis malonate and ketomalonate are more effective than maleate in inducing isomerase synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.1 Alcaligenes faecalis
-
-
-
5.2.1.1 Pseudomonas fluorescens
-
-
-
5.2.1.1 Pseudomonas sp.
-
-
-
5.2.1.2 Vibrio sp.
-
-
-
5.3.3.7 Pseudomonas putida
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.3.7 trans-aconitate = cis-aconitate a single catalytic base is found on the enzyme surface, the mechanism involves deprotonation to form a carbanion Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.1 Maleate
-
 Alcaligenes faecalis Fumarate
-
 ?
5.2.1.1 Maleate
-
 Pseudomonas fluorescens Fumarate
-
 ?
5.2.1.1 Maleate
-
 Pseudomonas sp. Fumarate
-
 ?
5.2.1.1 additional information malonate and ketomalonate are more effective than maleate in inducing isomerase synthesis Alcaligenes faecalis ?
-
 ?
5.2.1.2 Maleylacetoacetate + GSH Vibrio sp. Fumarylacetoacetate + GSSG ?
5.3.3.7 trans-Aconitate r Pseudomonas putida cis-Aconitate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.2.1.1
-
 25 pH 6, maximal stability in presence of small quantities of dithiothreitol Alcaligenes faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2.1.2 additional information
-
 additional information
-
 Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.1 8.3
-
-
 Alcaligenes faecalis
5.2.1.1 8.4
-
-
 Pseudomonas fluorescens
5.2.1.1 8.4
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.2.1.1 6.7 9.4
-
 Pseudomonas fluorescens
5.2.1.1 6.7 9.4 about 50% of maximal activity at pH 6.7 and pH 9.4 Pseudomonas sp.
5.2.1.1 6.8 9.3 about 50% of maximal activity at pH 6.8 and 9.3 Alcaligenes faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.2.1.1 5 7 30°C, rapid loss of activity below pH 5 and above pH 7 Alcaligenes faecalis
5.2.1.1 6
-
 0°C-25°C, maximal stability in presence of small quantities of dithiothreitol Alcaligenes faecalis