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Literature summary extracted from

  • Takahashi, K.; Moore, S.
    Ribonuclease T1 (1982), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 15, 435-468.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.24 Aspergillus oryzae Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.24 Cu2+
-
 Aspergillus oryzae
4.6.1.24 iodoacetate
-
 Aspergillus oryzae
4.6.1.24 Tosylglycolate protection by substrate analogs Aspergillus oryzae
4.6.1.24 Zn2+
-
 Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.24 additional information
-
 additional information
-
 Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.24 extracellular
-
 Aspergillus oryzae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.24 11085
-
 primary structure, Aspergillus oryzae, 1 * 11085 Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.24 RNA + H2O Aspergillus oryzae two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.24 Aspergillus oryzae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.24 commercial preparation Taka-diastase, commercial product of Aspergillus oryzae Aspergillus oryzae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.24 GpA + H2O
-
 Aspergillus oryzae guanosine 3'-phosphate + adenosine
-
 ?
4.6.1.24 GpG + H2O
-
 Aspergillus oryzae guanosine 3'-phosphate + guanosine
-
 ?
4.6.1.24 guanosine 2',3'-cyclic phosphate + H2O
-
 Aspergillus oryzae guanosine-3'-phosphate
-
 ?
4.6.1.24 RNA + H2O essential requirements: keto group at 6 position, trivalent nitrogen at 7 position of purine base Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ?
4.6.1.24 RNA + H2O first step (r): cleavage of internucleotide bonds between 3'-guanylic acid groups and the 5'-hydroxyl groups of the adjacent nucleotides with the intermediary formation of guanosine 2',3'-cyclic phosphate, second step (ir): hydrolysis of cyclic phosphate to produce 3'-guanylate Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ?
4.6.1.24 RNA + H2O specificity Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ?
4.6.1.24 RNA + H2O two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates Aspergillus oryzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.24 monomer primary structure, Aspergillus oryzae, 1 * 11085 Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6.1.24 38
-
 GpU
-
 Aspergillus oryzae
4.6.1.24 62
-
 GpG
-
 Aspergillus oryzae
4.6.1.24 96
-
 GpA
-
 Aspergillus oryzae
4.6.1.24 350
-
 GpC
-
 Aspergillus oryzae