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Literature summary extracted from
- Microbial ribonucleases with special reference to RNases T1, T2, N1, and U2 (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 205-250.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.6.1.24 | Proteases, fairly resistant to proteases: carboxypeptidase A, leucine aminopeptidase, trypsin, chymotrypsin <1>, resistant to trypsin and chymotrypsin, sensitive to pepsin | Aspergillus oryzae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.19 | 2'-AMP | - |
Aspergillus oryzae |  |
| 4.6.1.19 | 2'-CMP | - |
Aspergillus oryzae | |
| 4.6.1.19 | 2'-GMP | - |
Aspergillus oryzae | |
| 4.6.1.19 | 3'-AMP | - |
Aspergillus oryzae | |
| 4.6.1.19 | AgNO3 | - |
Aspergillus oryzae | |
| 4.6.1.19 | Cu2+ | - |
Aspergillus oryzae | |
| 4.6.1.19 | Fe2+ | - |
Aspergillus oryzae | |
| 4.6.1.19 | Hg2+ | - |
Aspergillus oryzae | |
| 4.6.1.19 | MgCl2 | slight | Aspergillus oryzae | |
| 4.6.1.19 | Mn2+ | - |
Aspergillus oryzae | |
| 4.6.1.19 | Na2S | - |
Aspergillus oryzae | |
| 4.6.1.19 | NaCl | - |
Aspergillus oryzae | |
| 4.6.1.19 | Zn2+ | - |
Aspergillus oryzae | |
| 4.6.1.24 | Ag+ | - |
Aspergillus oryzae | |
| 4.6.1.24 | Cu2+ | - |
Aspergillus oryzae | |
| 4.6.1.24 | Fe2+ | - |
Aspergillus oryzae | |
| 4.6.1.24 | Glyoxal | - |
Aspergillus oryzae | |
| 4.6.1.24 | Hg2+ | - |
Aspergillus oryzae | |
| 4.6.1.24 | iodoacetate | - |
Aspergillus oryzae | |
| 4.6.1.24 | Mg2+ | - |
Aspergillus oryzae | |
| 4.6.1.24 | Mn2+ | - |
Aspergillus oryzae | |
| 4.6.1.24 | Mononucleotides | e.g. 2'-GMP, 3'-GMP, 5'-GMP, 3'-CMP, 2'(3')-UMP | Aspergillus oryzae | |
| 4.6.1.24 | additional information | fairly resistant to proteases, e.g. carboxypeptidase A, leucine aminopeptidase, trypsin, chymotrypsin | Aspergillus oryzae | |
| 4.6.1.24 | Na2S | - |
Aspergillus oryzae | |
| 4.6.1.24 | Phenylglyoxal | - |
Aspergillus oryzae | |
| 4.6.1.24 | substrate analogs | - |
Aspergillus oryzae | |
| 4.6.1.24 | Zn2+ | - |
Aspergillus oryzae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.6.1.19 | 0.23 | - |
2',3'-cAMP | - |
Aspergillus oryzae | |
| 4.6.1.19 | 0.4 | - |
2',3'-cCMP | - |
Aspergillus oryzae | |
| 4.6.1.19 | 6.1 | - |
2',3'-cUMP | - |
Aspergillus oryzae | |
| 4.6.1.19 | 6.5 | - |
2',3'-cGMP | - |
Aspergillus oryzae | |
| 4.6.1.24 | additional information | - |
additional information | pH-dependence of Km | Aspergillus oryzae | |
| 4.6.1.24 | 0.0238 | - |
GpU | - |
Aspergillus oryzae | |
| 4.6.1.24 | 0.0268 | - |
GpA | - |
Aspergillus oryzae | |
| 4.6.1.24 | 0.0291 | - |
GpG | - |
Aspergillus oryzae | |
| 4.6.1.24 | 0.9445 | - |
GpC | - |
Aspergillus oryzae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.6.1.20 | extracellular | - |
Ustilago sphaerogena | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 36200 | - |
amino acid analysis | Aspergillus oryzae |
| 4.6.1.20 | 10000 | - |
Ustilago sphaerogena, amino acid analysis | Ustilago sphaerogena |
| 4.6.1.24 | 11085 | - |
primary structure, Aspergillus oryzae, 1 * 11085 | Aspergillus oryzae |
| 4.6.1.24 | 11090 | - |
Aspergillus oryzae, amino acid analysis | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.20 | RNA + H2O | Ustilago sphaerogena | - |
? | - |
? | |
| 4.6.1.24 | RNA + H2O | Aspergillus oryzae | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.6.1.19 | Aspergillus oryzae | - |
- |
- |
| 4.6.1.20 | Ustilago sphaerogena | - |
smut fungus | - |
| 4.6.1.24 | Aspergillus oryzae | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.6.1.24 | Photooxidation, in presence of Methylene Blue, riboflavine or Rose Bengal inactivation | Aspergillus oryzae |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.6.1.19 | glycoprotein | 12-15% carbohydrate, RNase T2-A: mannose, glucose, RNase T2-B: mannose galactose | Aspergillus oryzae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.6.1.19 | - |
Aspergillus oryzae |
| 4.6.1.20 | - |
Ustilago sphaerogena |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.6.1.19 | RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates: first step reversible cleavage of phosphodiester bond between 3'-adenylic acid group and 5'-hydroxyl group at adjacent nucleotides in RNA chain with formation of nucleoside 2',3'-cyclic phosphates and oligonucleotides with 2',3'-cyclic phosphate at 3'-terminal, second step: hydrolysis of terminal cyclic phosphate groups with formation of corresponding 3'-phosphates | Aspergillus oryzae | |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] | mechanism | Aspergillus oryzae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.6.1.19 | commercial preparation | Takadiastase powder | Aspergillus oryzae | - |
| 4.6.1.24 | commercial preparation | Taka-diastase, commercial product of Aspergillus oryzae | Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | additional information | - |
- |
Aspergillus oryzae |
| 4.6.1.24 | additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.19 | 2',3'-cAMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 4.6.1.19 | 2',3'-cCMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 4.6.1.19 | 2',3'-cGMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 4.6.1.19 | 2',3'-cUMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 4.6.1.19 | RNA + H2O | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates, first step reversible cleavage of phosphodiester bond between 3'-adenylic acid group and 5'-hydroxyl group at adjacent nucleotides in RNA chain with formation of nucleoside 2',3'-cyclic phosphates and oligonucleotides with 2',3'-cyclic phosphate at 3'-terminal, second step: hydrolysis of terminal cyclic phosphate groups with formation of corresponding 3'-phosphates, no base specificity | Aspergillus oryzae | 3'-phosphomononucleotides | - |
? | |
| 4.6.1.20 | RNA + H2O | r, preferential cleavage at Ap or Gp | Ustilago sphaerogena | 3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates | - |
? | |
| 4.6.1.20 | RNA + H2O | - |
Ustilago sphaerogena | ? | - |
? | |
| 4.6.1.24 | GpA + H2O | - |
Aspergillus oryzae | guanosine 3'-phosphate + adenosine | - |
? | |
| 4.6.1.24 | GpG + H2O | - |
Aspergillus oryzae | guanosine 3'-phosphate + guanosine | - |
? | |
| 4.6.1.24 | guanosine 2',3'-cyclic phosphate + H2O | - |
Aspergillus oryzae | guanosine-3'-phosphate | - |
? | |
| 4.6.1.24 | additional information | synthesis of guanylylnucleosides, oligoguanylate, guanosine-containing oligonucleotides with (3'-5')phosphodiester bonds, synthetic reaction can lead to the formation of unnatural (2'-5')-phosphodiester bonds, not: DNA, L-guanosine 2',3'-cyclic phosphate, L-inosine 2',3'-cyclic phosphate | Aspergillus oryzae | ? | - |
? | |
| 4.6.1.24 | RNA + H2O | essential requirements: keto group at 6 position, trivalent nitrogen at 7 position of purine base | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| 4.6.1.24 | RNA + H2O | first step (r): cleavage of internucleotide bonds between 3'-guanylic acid groups and the 5'-hydroxyl groups of the adjacent nucleotides with the intermediary formation of guanosine 2',3'-cyclic phosphate, second step (ir): hydrolysis of cyclic phosphate to produce 3'-guanylate | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| 4.6.1.24 | RNA + H2O | specificity | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| 4.6.1.24 | RNA + H2O | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates | Aspergillus oryzae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.6.1.24 | monomer | primary structure, Aspergillus oryzae, 1 * 11085 | Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 80 | - |
pH 6.0, 5 min | Aspergillus oryzae |
| 4.6.1.20 | 80 | - |
unstable above | Ustilago sphaerogena |
| 4.6.1.24 | 100 | - |
10 min, pH 6 | Aspergillus oryzae |
| 4.6.1.24 | 100 | - |
above pH 9 unstable | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 4.5 | - |
RNA or homopolymer digestion | Aspergillus oryzae |
| 4.6.1.19 | 6 | 6.3 | hydrolysis of various nucleoside 2',3'-cyclic phosphates, degradation of 3'-nucleotide benzyl ester | Aspergillus oryzae |
| 4.6.1.20 | 4.5 | - |
RNA | Ustilago sphaerogena |
| 4.6.1.24 | 4.5 | - |
xanthosine 2',3'-cyclic phosphate | Aspergillus oryzae |
| 4.6.1.24 | 7.2 | - |
hydrolysis of guanosine 2',3'-cyclic phosphate | Aspergillus oryzae |
| 4.6.1.24 | 7.5 | - |
RNA digestion | Aspergillus oryzae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.24 | 4 | 8.5 | - |
Aspergillus oryzae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.19 | 6 | - |
80°C, 5 min | Aspergillus oryzae |
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