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Literature summary extracted from
- Purification and some properties of UDP-N-acetylglucosamine 4-epimerase from Bacillus subtilis (1985), Agric. Biol. Chem., 49, 603-609.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.3.7 | Cu2+ | - |
Bacillus subtilis |  |
| 5.1.3.7 | glucosamine | competitive with UDP-N-acetylglucosamine | Bacillus subtilis | |
| 5.1.3.7 | HgCl2 | - |
Bacillus subtilis | |
| 5.1.3.7 | iodoacetate | - |
Bacillus subtilis | |
| 5.1.3.7 | NEM | - |
Bacillus subtilis | |
| 5.1.3.7 | PCMB | - |
Bacillus subtilis | |
| 5.1.3.7 | Zn2+ | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.7 | 4.4 | - |
UDP-N-acetylglucosamine | - |
Bacillus subtilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.3.7 | Ca2+ | stimulates | Bacillus subtilis | |
| 5.1.3.7 | Co2+ | stimulates | Bacillus subtilis | |
| 5.1.3.7 | Mg2+ | stimulates | Bacillus subtilis | |
| 5.1.3.7 | Mn2+ | stimulates | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.7 | 62000 | - |
gel filtration | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.7 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.7 | - |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.7 | 565 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.7 | UDP-N-acetyl-D-glucosamine | - |
Bacillus subtilis | UDP-N-acetyl-D-galactosamine | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.7 | 30 | 35 | without NAD+ | Bacillus subtilis |
| 5.1.3.7 | 40 | 45 | in presence of 1 mM NAD+ | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.7 | 50 | - |
pH 7.5, 15 min, 65% loss of activity in presence of 1 mM NAD+, in absence of NAD+ complete loss of activity | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.7 | 7 | 9 | - |
Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.7 | 7.5 | 9 | 4°C, stable | Bacillus subtilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.3.7 | NAD+ | highly stimulated by small amounts, Km: 0.13 mM | Bacillus subtilis | |
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