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Literature summary extracted from
- Multiple forms of mutarotases from the kidney, liver, and small intestine of rats: purification, properties, subcellular localization and developmental changes (1983), J. Biochem., 94, 421-431.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.3 | 2 | - |
alpha-D-Fucose | alpha-D-xylose, enzyme form II from kidney and liver | Rattus norvegicus |  |
| 5.1.3.3 | 5 | - |
alpha-D-glucose | alpha-D-xylose, enzyme form I and II from small intestine | Rattus norvegicus | |
| 5.1.3.3 | 8 | - |
alpha-D-xylose | enzyme form IV from liver | Rattus norvegicus | |
| 5.1.3.3 | 9 | - |
alpha-D-xylose | enzyme form IV from kidney | Rattus norvegicus | |
| 5.1.3.3 | 10 | - |
alpha-D-galactose | enzyme form III from kidney, enzyme form I and II from small intestine | Rattus norvegicus | |
| 5.1.3.3 | 11 | - |
alpha-D-galactose | enzyme form IV from kidney | Rattus norvegicus | |
| 5.1.3.3 | 12 | - |
alpha-D-glucose | beta-L-arabinose, enzyme form I from kidney, enzyme form III from liver | Rattus norvegicus | |
| 5.1.3.3 | 12 | - |
alpha-D-glucose | alpha-D-galactose, enzyme form I from kidney | Rattus norvegicus | |
| 5.1.3.3 | 13 | - |
D-fucose | alpha-D-galactose, enzyme form IV from liver | Rattus norvegicus | |
| 5.1.3.3 | 13 | - |
D-fucose | beta-L-arabinose, enzyme form III from kidney | Rattus norvegicus | |
| 5.1.3.3 | 14 | - |
alpha-D-galactose | alpha-D-xylose, enzyme form I2 from liver, enzyme form I from kidney | Rattus norvegicus | |
| 5.1.3.3 | 14 | - |
alpha-D-galactose | enzyme form III from liver | Rattus norvegicus | |
| 5.1.3.3 | 15 | - |
D-glucose | beta-L-arabinose, enzyme form IV from liver, enzyme form I and II from small intestine | Rattus norvegicus | |
| 5.1.3.3 | 15 | - |
D-glucose | alpha-D-galactose, enzyme form II from kidney, enzyme form I1 from liver | Rattus norvegicus | |
| 5.1.3.3 | 15 | - |
D-glucose | alpha-D-xylose, enzyme form I1 from liver | Rattus norvegicus | |
| 5.1.3.3 | 16 | - |
alpha-D-galactose | enzyme form II from liver | Rattus norvegicus | |
| 5.1.3.3 | 17 | - |
alpha-D-galactose | beta-L-arabinose, enzyme form II from kidney, enzyme form I1 from liver | Rattus norvegicus | |
| 5.1.3.3 | 17 | - |
alpha-D-galactose | alpha-D-xylose, enzyme form III from kidney and liver | Rattus norvegicus | |
| 5.1.3.3 | 17 | - |
alpha-D-galactose | enzyme form I2 from liver | Rattus norvegicus | |
| 5.1.3.3 | 18 | - |
beta-D-arabinose | enzyme form I2 from liver | Rattus norvegicus | |
| 5.1.3.3 | 19 | - |
alpha-D-glucose | beta-L-arabinose, enzyme form II from liver | Rattus norvegicus | |
| 5.1.3.3 | 30 | - |
alpha-D-glucose | enzyme form I from kiney, enzyme form I1 from liver, enzyme form IV from liver | Rattus norvegicus | |
| 5.1.3.3 | 31 | - |
alpha-D-glucose | enzyme form I2 from liver, enzyme form I and II from small intestine | Rattus norvegicus | |
| 5.1.3.3 | 33 | - |
alpha-D-glucose | enzyme form IV from kidney, enzyme form III from liver | Rattus norvegicus | |
| 5.1.3.3 | 34 | - |
alpha-D-glucose | enzyme form II from kidney and liver | Rattus norvegicus | |
| 5.1.3.3 | 35 | - |
alpha-D-glucose | enzyme form II from kidney | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.1.3.3 | nucleus | of liver parenchymal cells and small intestine mucosal cells | Rattus norvegicus | 5634 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.3 | 41000 | - |
x * 41000, enzyme form II from kidney and liver, SDS-PAGE | Rattus norvegicus |
| 5.1.3.3 | 41300 | - |
x * 41300, enzyme form I1 and I2 from liver, SDS-PAGE | Rattus norvegicus |
| 5.1.3.3 | 41800 | - |
x * 41800, enzyme form I from kidney, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.3 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.3 | enzyme form I and II from kidney and enzyme form II from liver | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.1.3.3 | kidney | adult animals, 4 enzyme forms: I, II, III, and IV. Enzyme activity increases gradually after birth and reaches a maximum near adult level within 20 days | Rattus norvegicus | - |
| 5.1.3.3 | liver | parenchymal cells, adult animals, 4 enzyme forms: I, II, III, and IV. Enzyme form I is separated into type I1 and I2. Enzyme activity increases gradually after birth and reaches a maximum near adult level within 20 days | Rattus norvegicus | - |
| 5.1.3.3 | small intestine | mucosal cells, 10-day-old rats: 4 enzyme forms, I-IV. Adult rats: 2 enzyme forms, I and II, enzyme activity in suckling rats is several times higher than that of adult rats | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.3 | 16.3 | - |
enzyme form I, small intestine | Rattus norvegicus |
| 5.1.3.3 | 35.8 | - |
enzyme form II, small intestine | Rattus norvegicus |
| 5.1.3.3 | 510 | - |
enzyme form II, kidney | Rattus norvegicus |
| 5.1.3.3 | 512 | - |
enzyme form I, kidney | Rattus norvegicus |
| 5.1.3.3 | 709 | - |
enzyme form II, liver | Rattus norvegicus |
| 5.1.3.3 | 720 | - |
enzyme form I1, liver | Rattus norvegicus |
| 5.1.3.3 | 745 | - |
enzyme form I2, liver | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.3 | alpha-D-fucose | - |
Rattus norvegicus | beta-D-fucose | - |
? | |
| 5.1.3.3 | alpha-D-galactose | - |
Rattus norvegicus | beta-D-galactose | - |
? | |
| 5.1.3.3 | alpha-D-glucose | - |
Rattus norvegicus | beta-D-glucose | - |
? | |
| 5.1.3.3 | alpha-D-xylose | - |
Rattus norvegicus | beta-D-xylose | - |
? | |
| 5.1.3.3 | alpha-L-arabinose | - |
Rattus norvegicus | beta-L-arabinose | - |
? | |
| 5.1.3.3 | beta-D-arabinose | - |
Rattus norvegicus | alpha-D-arabinose | - |
r | |
| 5.1.3.3 | D-Fucose | - |
Rattus norvegicus | ? | - |
? | |
| 5.1.3.3 | D-glucose | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.3 | ? | x * 41000, enzyme form II from kidney and liver, SDS-PAGE | Rattus norvegicus |
| 5.1.3.3 | ? | x * 41800, enzyme form I from kidney, SDS-PAGE | Rattus norvegicus |
| 5.1.3.3 | ? | x * 41300, enzyme form I1 and I2 from liver, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.3 | 35 | 38 | - |
Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.3 | 50 | - |
stable up to, enzyme form I and II from kidney liver and small instetine | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.3 | 7 | 7.5 | enzyme form I and II from kidney, enzyme form I2 and II from liver, enzyme form I and II from small intestine | Rattus norvegicus |
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