Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vorgias, C.E.; Lemaire, H.G.; Wilson, K.S.
    Overexpression and purification of the galactose operon enzymes from Escherichia coli (1991), Protein Expr. Purif., 2, 330-338.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 overexpression in Escherichia coli Escherichia coli
5.1.3.2
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.2 0.2
-
 UDPgalactose
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.2 38000
-
 2 * 38000, SDS-PAGE Escherichia coli
5.1.3.2 80000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Escherichia coli
-
-
-
5.1.3.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6
-
 Escherichia coli
5.1.3.2
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + D-galactose
-
 Escherichia coli ADP + alpha-D-galactose 1-phosphate
-
 ?
5.1.3.2 UDP-galactose
-
 Escherichia coli UDP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.2 dimer 2 * 38000, SDS-PAGE Escherichia coli