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Literature summary extracted from

  • Lee, L.J.; Kimura, A.; Tochikura, T.
    Purification and properties of UDP-galactose 4-epimerase from Bifidobacterium bifidum (1978), Agric. Biol. Chem., 42, 731-737.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.2 fructose 4-phosphate stimulates Bifidobacterium bifidum
5.1.3.2 fructose 6-phosphate stimulates Bifidobacterium bifidum
5.1.3.2 Galactose 1-phosphate stimulates Bifidobacterium bifidum
5.1.3.2 glucose 1-phosphate stimulates Bifidobacterium bifidum
5.1.3.2 glucose 6-phosphate stimulates Bifidobacterium bifidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.2 fructose 1,6-diphosphate inhibition is enhanced by combination with UMP Bifidobacterium bifidum
5.1.3.2 Galactose plus UMP strong inhibition in the presence of UMP, no inhibition by UMP or sugar alone Bifidobacterium bifidum
5.1.3.2 glucose plus UMP strong inhibition in the presence of UMP, no inhibition by UMP or sugar alone Bifidobacterium bifidum
5.1.3.2 HgCl2
-
 Bifidobacterium bifidum
5.1.3.2 NEM
-
 Bifidobacterium bifidum
5.1.3.2 p-chloromercuribenzoate
-
 Bifidobacterium bifidum
5.1.3.2 UDP
-
 Bifidobacterium bifidum
5.1.3.2 UTP slightly Bifidobacterium bifidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.2 0.53
-
 UDPgalactose
-
 Bifidobacterium bifidum
5.1.3.2 1.4
-
 UDPglucose
-
 Bifidobacterium bifidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.2 45000
-
 2 * 45000, SDS-PAGE Bifidobacterium bifidum
5.1.3.2 90000
-
 gel filtration Bifidobacterium bifidum

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Bifidobacterium bifidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.2
-
 Bifidobacterium bifidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.2 41.8
-
-
 Bifidobacterium bifidum

Storage Stability

EC Number Storage Stability Organism
5.1.3.2 4°C or -20°C, 0.2 mg/ml enzyme concentration, 0.01 M potassium phosphate buffer, pH 7.5. About 60% of the initial activity remains for 3 months at 4°C without any protector, and in the presence of 20% glycerol more than 80% of the activity remains Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 UDP-galactose
-
 Bifidobacterium bifidum UDP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.2 dimer 2 * 45000, SDS-PAGE Bifidobacterium bifidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.2 8 9
-
 Bifidobacterium bifidum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.2 7 9.7 7: about 50% of maximal activity, 9.7: about 40% of maximal activity Bifidobacterium bifidum