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Literature summary extracted from

  • Stäsche, R.; Hinderlich, S.; Weise, C.; Effertz, K.; Lucka, L.; Moormann, P.; Reutter, W.
    A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Molecular cloning and functional expression of UDP-N-acetyl-glucosamine 2-epimerase/N-acetylmannosamine kinase (1997), J. Biol. Chem., 272, 24319-24324.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.183 recombinant bifunctional enzyme is expressed in COS7 cells Rattus norvegicus
5.1.3.14 bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60, expressed in COS7 cells Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.183 cytosol
-
 Rattus norvegicus 5829
-
5.1.3.14 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.183 79000
-
 calculated from cDNA Rattus norvegicus
5.1.3.14 79000
-
 x * 79000, calculation from nucleotide sequence Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.183 Rattus norvegicus
-
-
-
5.1.3.14 Rattus norvegicus O35826 bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.183
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.183 intestinal mucosa
-
 Rattus norvegicus
-
3.2.1.183 liver
-
 Rattus norvegicus
-
3.2.1.183 salivary gland
-
 Rattus norvegicus
-
5.1.3.14 intestinal mucosa
-
 Rattus norvegicus
-
5.1.3.14 liver
-
 Rattus norvegicus
-
5.1.3.14 salivary gland enzyme is detected only in sialoglycoprotein-secreting tissues Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.183 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Rattus norvegicus N-acetyl-alpha-D-mannosamine + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.14 ? x * 79000, calculation from nucleotide sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.183 UDP-GlcNAc 2-epimerase bifunctional enzyme exhibiting UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activity Rattus norvegicus
3.2.1.183 UDP-N-acetylglucosamine 2-epimerase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.183 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.183 8.5
-
 assay at Rattus norvegicus