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Literature summary extracted from
- Purification, properties and in situ localization of the amphibolic enzymes D-ribulose 5-phosphate 3-epimerase and transketolase from spinach chloroplasts (1989), Eur. J. Biochem., 252, 237-244.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.2.1.1 | 0.067 | - |
D-xylulose 5-phosphate | - |
Spinacia oleracea |  |
| 2.2.1.1 | 0.33 | - |
D-ribose 5-phosphate | - |
Spinacia oleracea | |
| 5.1.3.1 | 0.25 | - |
D-ribulose 5-phosphate | - |
Spinacia oleracea | |
| 5.1.3.1 | 0.25 | - |
D-ribulose 5-phosphate | pH 7.5 | Spinacia oleracea | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.2.1.1 | chloroplast | - |
Spinacia oleracea | 9507 | - |
| 5.1.3.1 | chloroplast | stroma, 90% of the total epimerase is associated with thylakoid membranes | Spinacia oleracea | 9507 | - |
| 5.1.3.1 | chloroplast | stroma | Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | 74000 | - |
alpha2, 2 * 74000, SDS-PAGE | Spinacia oleracea |
| 2.2.1.1 | 160000 | - |
gel filtration | Spinacia oleracea |
| 5.1.3.1 | 23000 | - |
8 * 23000, SDS-PAGE | Spinacia oleracea |
| 5.1.3.1 | 180000 | - |
gel filtration | Spinacia oleracea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.2.1.1 | Spinacia oleracea | - |
spinach | - |
| 5.1.3.1 | Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.2.1.1 | - |
Spinacia oleracea |
| 5.1.3.1 | - |
Spinacia oleracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.1.3.1 | leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.2.1.1 | 12 | - |
- |
Spinacia oleracea |
| 5.1.3.1 | additional information | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.2.1.1 | D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Spinacia oleracea | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
| 5.1.3.1 | D-Ribulose 5-phosphate | - |
Spinacia oleracea | D-Xylulose 5-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.2.1.1 | dimer | alpha2, 2 * 74000, SDS-PAGE | Spinacia oleracea |
| 5.1.3.1 | octamer | 8 * 23000, SDS-PAGE | Spinacia oleracea |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.2.1.1 | 0.0148 | - |
D-ribose 5-phosphate | - |
Spinacia oleracea | |
| 2.2.1.1 | 0.0187 | - |
Xylulose 5-phosphate | - |
Spinacia oleracea | |
| 5.1.3.1 | 0.138 | - |
D-ribulose 5-phosphate | - |
Spinacia oleracea | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.1 | 8.3 | - |
- |
Spinacia oleracea |
| 5.1.3.1 | 8.3 | - |
reaction with D-ribulose 5-phosphate | Spinacia oleracea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.1 | 6.5 | 9 | pH 6.5: about 90% of maximal activity, pH 9.0: 95% of maximal activity | Spinacia oleracea |
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