BRENDA - Enzyme Database

Purification and mechanism of action of proline racemase

Cardinale, G.J.; Abeles, R.H.; Biochemistry 7, 3970-3978 (1968)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.1.1.4
2-mercaptoethanol
activates; optimal concentration: 0.02 M
Acetoanaerobium sticklandii
5.1.1.4
additional information
addition of pyridoxal 5'-phosphate does not increase activity
Acetoanaerobium sticklandii
5.1.1.4
NaBH4
activates
Acetoanaerobium sticklandii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.1.4
1,10-phenanthroline
-
Acetoanaerobium sticklandii
5.1.1.4
2-Furoic acid
weak
Acetoanaerobium sticklandii
5.1.1.4
2-Thiophenecarboxylic acid
-
Acetoanaerobium sticklandii
5.1.1.4
alpha-Pipecoline
-
Acetoanaerobium sticklandii
5.1.1.4
Asp
-
Acetoanaerobium sticklandii
5.1.1.4
Fumaric acid
-
Acetoanaerobium sticklandii
5.1.1.4
HPO42-
no inhibition by H2PO4-
Acetoanaerobium sticklandii
5.1.1.4
iodoacetamide
-
Acetoanaerobium sticklandii
5.1.1.4
iodoacetate
DL-Pro and pyrrole-2-carboxylate can protect
Acetoanaerobium sticklandii
5.1.1.4
Maleic acid
-
Acetoanaerobium sticklandii
5.1.1.4
malonic acid
-
Acetoanaerobium sticklandii
5.1.1.4
Methoxyacetic acid
weak
Acetoanaerobium sticklandii
5.1.1.4
Phthalic acid
-
Acetoanaerobium sticklandii
5.1.1.4
pipecolic acid
weak
Acetoanaerobium sticklandii
5.1.1.4
pyrrole-2-carboxylic acid
-
Acetoanaerobium sticklandii
5.1.1.4
sarcosine
-
Acetoanaerobium sticklandii
5.1.1.4
succinic acid
-
Acetoanaerobium sticklandii
5.1.1.4
Tetrahydrofuroic acid
weak
Acetoanaerobium sticklandii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.1.4
2.3
-
L-Pro
-
Acetoanaerobium sticklandii
5.1.1.4
3.8
-
D-Pro
-
Acetoanaerobium sticklandii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.1.1.4
Acetoanaerobium sticklandii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.1.1.4
-
Acetoanaerobium sticklandii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1.1.4
600
-
-
Acetoanaerobium sticklandii
Storage Stability
EC Number
Storage Stability
Organism
5.1.1.4
stored 6 months in the refrigerator without significant loss of activity
Acetoanaerobium sticklandii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.1.4
L-Pro
-
2122
Acetoanaerobium sticklandii
D-Pro
-
2122
Acetoanaerobium sticklandii
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.1.1.4
70
-
half-life: 3.6 min, in D2O 17 min
Acetoanaerobium sticklandii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.1.1.4
2-mercaptoethanol
activates; optimal concentration: 0.02 M
Acetoanaerobium sticklandii
5.1.1.4
additional information
addition of pyridoxal 5'-phosphate does not increase activity
Acetoanaerobium sticklandii
5.1.1.4
NaBH4
activates
Acetoanaerobium sticklandii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.1.1.4
1,10-phenanthroline
-
Acetoanaerobium sticklandii
5.1.1.4
2-Furoic acid
weak
Acetoanaerobium sticklandii
5.1.1.4
2-Thiophenecarboxylic acid
-
Acetoanaerobium sticklandii
5.1.1.4
alpha-Pipecoline
-
Acetoanaerobium sticklandii
5.1.1.4
Asp
-
Acetoanaerobium sticklandii
5.1.1.4
Fumaric acid
-
Acetoanaerobium sticklandii
5.1.1.4
HPO42-
no inhibition by H2PO4-
Acetoanaerobium sticklandii
5.1.1.4
iodoacetamide
-
Acetoanaerobium sticklandii
5.1.1.4
iodoacetate
DL-Pro and pyrrole-2-carboxylate can protect
Acetoanaerobium sticklandii
5.1.1.4
Maleic acid
-
Acetoanaerobium sticklandii
5.1.1.4
malonic acid
-
Acetoanaerobium sticklandii
5.1.1.4
Methoxyacetic acid
weak
Acetoanaerobium sticklandii
5.1.1.4
Phthalic acid
-
Acetoanaerobium sticklandii
5.1.1.4
pipecolic acid
weak
Acetoanaerobium sticklandii
5.1.1.4
pyrrole-2-carboxylic acid
-
Acetoanaerobium sticklandii
5.1.1.4
sarcosine
-
Acetoanaerobium sticklandii
5.1.1.4
succinic acid
-
Acetoanaerobium sticklandii
5.1.1.4
Tetrahydrofuroic acid
weak
Acetoanaerobium sticklandii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.1.1.4
2.3
-
L-Pro
-
Acetoanaerobium sticklandii
5.1.1.4
3.8
-
D-Pro
-
Acetoanaerobium sticklandii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.1.1.4
-
Acetoanaerobium sticklandii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1.1.4
600
-
-
Acetoanaerobium sticklandii
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
5.1.1.4
stored 6 months in the refrigerator without significant loss of activity
Acetoanaerobium sticklandii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.1.1.4
L-Pro
-
2122
Acetoanaerobium sticklandii
D-Pro
-
2122
Acetoanaerobium sticklandii
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
5.1.1.4
70
-
half-life: 3.6 min, in D2O 17 min
Acetoanaerobium sticklandii