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Literature summary extracted from

  • Nakajima, N.; Tanizawa, K.; Tanaka, H.; Soda, K.
    Distribution of glutamate racemase in lactic acid bacteria and further characterization of the enzyme from Pediococcus pentosaceus (1988), Agric. Biol. Chem., 52, 3099-3104.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.3 2-Nitro-5-thiocyanobenzoate complete inactivation at 0.1 mM Pediococcus pentosaceus
5.1.1.3 iodoacetamide
-
 Pediococcus pentosaceus
5.1.1.3 L-alpha-aminohexanedioate
-
 Pediococcus pentosaceus
5.1.1.3 Thiol-blocking reagents
-
 Pediococcus pentosaceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.3 10
-
 L-Glu
-
 Pediococcus pentosaceus
5.1.1.3 14
-
 D-Glu
-
 Pediococcus pentosaceus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.3 L-Glu Lactic acid bacteria the biosynthesis of D-Glu, one of the essential components of bacterial cell-wall peptidoglycan, is catalyzed by glutamate racemase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.3 Lactic acid bacteria
-
 enzyme occurs exclusively in lactic acid bacteria
-
5.1.1.3 Lacticaseibacillus casei
-
 subsp. casei and subsp. rhamnosus
-
5.1.1.3 Lactiplantibacillus plantarum
-
-
-
5.1.1.3 Lactobacillus sp.
-
-
-
5.1.1.3 Leuconostoc lactis
-
-
-
5.1.1.3 Limosilactobacillus fermentum
-
-
-
5.1.1.3 no activity in Clostridium sp.
-
-
-
5.1.1.3 Pediococcus acidilactici
-
-
-
5.1.1.3 Pediococcus pentosaceus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.3
-
 Pediococcus pentosaceus

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.1.3 L-glutamate = D-glutamate initial step is the proton abstraction from the substrate alpha-carbon atom, which is mediated by an amino acid residue in the enzyme protein Pediococcus pentosaceus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.3 D-Glu
-
 Lactiplantibacillus plantarum L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Lacticaseibacillus casei L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Limosilactobacillus fermentum L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Pediococcus pentosaceus L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Lactobacillus sp. L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Lactic acid bacteria L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Pediococcus acidilactici L-Glu
-
 ?
5.1.1.3 D-Glu
-
 Leuconostoc lactis L-Glu
-
 ?
5.1.1.3 L-Glu the biosynthesis of D-Glu, one of the essential components of bacterial cell-wall peptidoglycan, is catalyzed by glutamate racemase Lactic acid bacteria ?
-
 ?
5.1.1.3 L-Homocysteinesulfinate slightly serves as substrate Pediococcus pentosaceus D-Homocysteinesulfinate
-
 ?