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Literature summary extracted from

  • Muramatsu, N.; Nosoh, Y.
    Some catalytic and molecular properties of threonine deaminase from Bacillus stearothermophilus (1996), J. Biochem., 80, 485-490.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.19 Ile more sensitive at 40 C than at 65 C Geobacillus stearothermophilus
4.3.1.19 L-Val slight inhibition Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.19 5.7
-
 L-Thr at 40°C Geobacillus stearothermophilus
4.3.1.19 13.9
-
 L-Thr at 65°C Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.19 49000
-
 4 * 49000, SDS-PAGE Geobacillus stearothermophilus
4.3.1.19 200000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19
-
 Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
4.3.1.19 4°C, pH 7.2 or 9.0, 24 h, complete inactivation after 7 days, stable in presence of egg albumin Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-threonine
-
 Geobacillus stearothermophilus 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.19 tetramer 4 * 49000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.19 65
-
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.19 9.2 9.6 at 40 C and 65 C Geobacillus stearothermophilus