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Literature summary extracted from

  • Sharma, R.K.; Mazumder, R.
    Purification, properties, and feedback control of L-threonine dehydratase from spinach (1970), J. Biol. Chem., 245, 3008-3014.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.19 L-Val activates Spinacia oleracea

General Stability

EC Number General Stability Organism
4.3.1.19 L-Ile protects the enzyme against inactivation at low temperatures Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.19 Ile feed-back inhibition; HgCl2 treated enzyme is less sensitive; L-Val partially reverses inhibition Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.19 K+ stimulates Spinacia oleracea
4.3.1.19 Li+ stimulates Spinacia oleracea
4.3.1.19 NH4+ stimulates Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.1.19 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.19 1
-
 L-Thr Spinacia oleracea
4.3.1.19 7.3
-
 L-Thr Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
4.3.1.19 4°C, little loss of activity after 50 days Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-serine
-
 Spinacia oleracea pyruvate + NH3
-
 ?
4.3.1.19 L-threonine
-
 Spinacia oleracea 2-oxobutanoate + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.19
-
-
 10 h, 81% loss of activity in presence of 0.05 mM Ile, stable in presence of 1 mM Ile Spinacia oleracea
4.3.1.19 27
-
 10 h, 27% loss of activity in presence of 0.05 mM Ile, stable in presence of 1 mM Ile Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.19 9
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.19 pyridoxal 5'-phosphate tightly bound Spinacia oleracea
4.3.1.19 pyridoxal 5'-phosphate cofactor Spinacia oleracea