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Literature summary extracted from

  • Oda, Y.; Nakano, Y.; Kitaoka, S.
    Occurence and some properties of two threonine dehydratases in Euglena gracilis (1983), J. Gen. Microbiol., 129, 57-61.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.19 additional information dehydratase I and II: not affected by adenylates Euglena gracilis

General Stability

EC Number General Stability Organism
4.3.1.19 loss of activity of dehydratase I at 37 C is prevented by 1 mM Ile, but that of dehydratase II is not Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.19 Ile dehydratase I, 50% inhibition at 0.35 mM Euglena gracilis
4.3.1.19 Leu dehydratase I, 6 mM, 50% inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.19 13
-
 L-Thr dehydratase II Euglena gracilis
4.3.1.19 34
-
 L-Thr dehydratase I Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.19 250000
-
 dehydratase II, gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Euglena gracilis
-
 dehydratase I and dehydratase II
-
4.3.1.19 Euglena gracilis SM-ZK
-
 dehydratase I and dehydratase II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19 partial Euglena gracilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.19 additional information
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-serine dehydratase I: 26% of the activity with L-Thr, dehydratase II: 16% of the activity with L-Thr Euglena gracilis pyruvate + NH3
-
 ?
4.3.1.19 L-serine dehydratase I: 26% of the activity with L-Thr, dehydratase II: 16% of the activity with L-Thr Euglena gracilis SM-ZK pyruvate + NH3
-
 ?
4.3.1.19 L-threonine
-
 Euglena gracilis 2-oxobutanoate + NH3
-
 ?
4.3.1.19 L-threonine
-
 Euglena gracilis SM-ZK 2-oxobutanoate + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.19 37
-
 10 min, 16% loss of activity of dehydratase I and 36% loss of activity of dehydratase II, loss of activity of dehydratase I is prevented by 1 mM Ile, but that of dehydratase II is not Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.19 8.5
-
 dehydratase II Euglena gracilis
4.3.1.19 9.5 11 dehydratase I Euglena gracilis