Any feedback?
Literature summary extracted from
- Purification, metal cofactor, N-terminal sequence and subunit composition of a 5-aminolevulinic acid dehydratase from the unicellular green alga Scenedesmus obliquus, mutant C-2A' (1996), Eur. J. Biochem., 236, 600-608.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.24 | EGTA prevents proteolysis | Tetradesmus obliquus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.24 | 0.26 | - |
5-aminolevulinate | - |
Tetradesmus obliquus |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.24 | Ca2+ | about 80% reactivation of the demetalled protein | Tetradesmus obliquus | |
| 4.2.1.24 | Co2+ | about 70% reactivation of the demetalled protein | Tetradesmus obliquus | |
| 4.2.1.24 | Fe2+ | about 70% reactivation of the demetalled protein | Tetradesmus obliquus | |
| 4.2.1.24 | Mg2+ | metal cofactor | Tetradesmus obliquus | |
| 4.2.1.24 | Mn2+ | can reactivate the demetallated protein | Tetradesmus obliquus | |
| 4.2.1.24 | Ni(2+) | about 70% reactivation of the demetalled protein | Tetradesmus obliquus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 42000 | - |
6 * 42000, SDS-PAGE | Tetradesmus obliquus |
| 4.2.1.24 | 282000 | - |
gel filtration | Tetradesmus obliquus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.24 | Tetradesmus obliquus | - |
mutant strain C-2A' | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.24 | - |
Tetradesmus obliquus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | additional information | - |
- |
Tetradesmus obliquus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.24 | 5-aminolevulinate + 5-aminolevulinate | - |
Tetradesmus obliquus | porphobilinogen + 2 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.24 | hexamer | 6 * 42000, SDS-PAGE | Tetradesmus obliquus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 8.3 | - |
- |
Tetradesmus obliquus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 9 | - |
highest stability at | Tetradesmus obliquus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
