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Literature summary extracted from

  • Bhosale, S.; Kshirsagar, D.; Pawar, P.; Yeole, T.; Ranade, D.
    Purification and characterization of 5-aminolevulinic acid dehydratase from Methanosarcina barkeri (1995), FEMS Microbiol. Lett., 127, 151-155.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.24 Cu2+
-
 Methanosarcina barkeri
4.2.1.24 Pb2+
-
 Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.24 K+ activates up to 3 mM Methanosarcina barkeri
4.2.1.24 Ni(2+) 0.15 mM, activates Methanosarcina barkeri
4.2.1.24 Zn2+ activates Methanosarcina barkeri
4.2.1.24 Zn2+ optimal stimulation at 0.1 mM Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.24 30000
-
 8 * 30000, SDS-PAGE Methanosarcina barkeri
4.2.1.24 280000
-
 gel filtration Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.24 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.24
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.24 0.39
-
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.24 5-aminolevulinate + 5-aminolevulinate
-
 Methanosarcina barkeri porphobilinogen + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.24 octamer 8 * 30000, SDS-PAGE Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.24 8.5
-
 two pH-optima at pH 8.5 and at pH 9.4 Methanosarcina barkeri
4.2.1.24 9.4
-
 two pH-optima at pH 8.5 and at pH 9.4 Methanosarcina barkeri