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Literature summary extracted from

  • Leuthner, B.; Leutwein, C.; Schultz,H.; Hörth, P.; Haehnel, W.; Schiltz, E.; Schägger, H.; Heider, J.
    Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalaysing the first step in anaerobic toluene metabolism (1998), Mol. Microbiol., 28, 615-628.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.11 expressed in Escherichia coli Thauera aromatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.11 10000
-
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE Thauera aromatica
4.1.99.11 12000
-
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE Thauera aromatica
4.1.99.11 90000
-
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE Thauera aromatica
4.1.99.11 94000
-
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE Thauera aromatica
4.1.99.11 220000
-
gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.11 toluene + fumarate Thauera aromatica
-
benzylsuccinate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.11 Thauera aromatica
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.99.11 rapidly inactivated by oxygen with a half-life of 20-30 s upon exposure to air Thauera aromatica

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.11 DEAE-Sepharose, hydroxyapatite, anion exchange chromatography, enzyme severely inactivated during purification Thauera aromatica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.11 0.0002
-
-
Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.11 toluene + fumarate
-
Thauera aromatica benzylsuccinate
-
?

Subunits

EC Number Subunits Comment Organism
4.1.99.11 tetramer alpha, alpha', beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE Thauera aromatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.11 8
-
-
Thauera aromatica

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.11 flavin indicated by fluorescence spectroscopy, chemical nature unknown Thauera aromatica