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Literature summary extracted from
- An archaebacterial ATPase, homologous to ATPases in the eukaryotic 26 S proteasome, activates protein breakdown by 20 S proteasomes (1999), J. Biol. Chem., 274, 26008-26014.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.1.5 | complete genome sequenced | Methanothermobacter thermautotrophicus |
| 5.6.1.5 | complete genome sequenced | Archaeoglobus fulgidus |
| 5.6.1.5 | complete genome sequenced | Methanosarcina thermophila |
| 5.6.1.5 | complete genome sequenced | Pyrococcus horikoshii |
| 5.6.1.5 | complete genome sequenced | Pyrobaculum aerophilum |
| 5.6.1.5 | pAMJHW03 amplified, putative ATPase cloning of S4 and expression of PAN in Escherichia coli Bl21(DE3) | Methanocaldococcus jannaschii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.1.5 | EDTA | - |
Methanocaldococcus jannaschii |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.1.5 | Mg2+ | - |
Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.6.1.5 | 50000 | - |
recombinant protein, expressed in E. coli, SDS-PAGE | Methanocaldococcus jannaschii |
| 5.6.1.5 | 650000 | - |
PAN, proteasome-activating nucleotidase, complex with ATPase activity, gel filtration | Methanocaldococcus jannaschii |
| 5.6.1.5 | 700000 | - |
- |
Saccharomyces cerevisiae |
| 5.6.1.5 | 700000 | - |
- |
Methanocaldococcus jannaschii |
| 5.6.1.5 | 700000 | - |
- |
Thermoplasma sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.1.5 | ATP + H2O + polypeptide | Homo sapiens | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Saccharomyces cerevisiae | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Methanothermobacter thermautotrophicus | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Trichormus variabilis | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Methanocaldococcus jannaschii | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Archaeoglobus fulgidus | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Methanosarcina thermophila | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Pyrococcus horikoshii | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Thermoplasma sp. | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | Pyrobaculum aerophilum | - |
ADP + phosphate + unfolded polypeptide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.1.5 | Archaeoglobus fulgidus | - |
- |
- |
| 5.6.1.5 | Homo sapiens | - |
- |
- |
| 5.6.1.5 | Methanocaldococcus jannaschii | - |
- |
- |
| 5.6.1.5 | Methanosarcina thermophila | - |
- |
- |
| 5.6.1.5 | Methanothermobacter thermautotrophicus | - |
- |
- |
| 5.6.1.5 | Pyrobaculum aerophilum | - |
- |
- |
| 5.6.1.5 | Pyrococcus horikoshii | - |
- |
- |
| 5.6.1.5 | Saccharomyces cerevisiae | - |
- |
- |
| 5.6.1.5 | Thermoplasma sp. | - |
acidophilum | - |
| 5.6.1.5 | Trichormus variabilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.1.5 | recombinant PAN, expressed in Escherichia coli Bl21(DE3) | Methanocaldococcus jannaschii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Homo sapiens | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Saccharomyces cerevisiae | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Methanothermobacter thermautotrophicus | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Trichormus variabilis | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Methanocaldococcus jannaschii | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Archaeoglobus fulgidus | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Methanosarcina thermophila | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Pyrococcus horikoshii | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Thermoplasma sp. | |
| 5.6.1.5 | ATP + H2O + polypeptide = ADP + phosphate + unfolded polypeptide | belongs to the AAA-type superfamily and, like EC 3.6.4.5, minus-end-directed kinesin ATPase, is involved in channel gating and polypeptide unfolding before proteolysis in the proteasome. Six ATPase subunits are present in the regulatory particle (RP) of 26S proteasome | Pyrobaculum aerophilum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Homo sapiens | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Saccharomyces cerevisiae | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Methanothermobacter thermautotrophicus | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Trichormus variabilis | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Archaeoglobus fulgidus | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Methanosarcina thermophila | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Pyrococcus horikoshii | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Thermoplasma sp. | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | ATP + H2O + polypeptide | - |
Pyrobaculum aerophilum | ADP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | CTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | CDP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | GTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | GDP + phosphate + unfolded polypeptide | - |
? | |
| 5.6.1.5 | UTP + H2O + polypeptide | - |
Methanocaldococcus jannaschii | UDP + phosphate + unfolded polypeptide | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.1.5 | proteasome S4 ATPase | - |
Homo sapiens |
| 5.6.1.5 | proteasome S4 ATPase | - |
Saccharomyces cerevisiae |
| 5.6.1.5 | proteasome S4 ATPase | - |
Methanocaldococcus jannaschii |
| 5.6.1.5 | proteasome S4 ATPase | - |
Thermoplasma sp. |
| 5.6.1.5 | proteasome-activating nucleotidase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.6.1.5 | 73 | - |
- |
Methanocaldococcus jannaschii |
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