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Literature summary extracted from

  • Shimizu, T.; Toyoshima, Y.Y.; Edamatsu, M.; Vale, R.D.
    Comparison of the motile and enzymatic properties of two microtubule minus-end-directed motors, ncd and cytoplasmic dynein (1995), Biochemistry, 34, 1575-1582.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.1.2 microtubule in presence of microtubule, dynein ATPase activity is stimulated by ca. 6fold Bos taurus
5.6.1.4 microtubule in presence of microtubule, ncd ATPase activity is stimulated by ca. 30fold Drosophila sp. (in: flies)
5.6.1.4 microtubules stimulates ncd ATPase activity by ca. 30fold Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.4 PCR from a ncd cDNA, ncd cloned, polypeptide expressed in Escherichia coli Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.2 AlCl3 1 mM, complete inhibition Bos taurus
5.6.1.2 vanadate dynein is cleaved by UV-vanadate treatment Bos taurus
5.6.1.4 additional information in contrast to cytoplasmic dynein, ncd polypeptide is not cleaved by UV-vanadate treatment, and its ATPase and motility are unaffected by vanadate (up to 0.1 mM) Drosophila sp. (in: flies)
5.6.1.4 V5+ dynein Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.1.2 0.033
-
 ATP
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.2 cytoplasm
-
 Bos taurus 5737
-
5.6.1.4 cytoplasm
-
 Bos taurus 5737
-
5.6.1.4 microtubule
-
 Drosophila sp. (in: flies) 5874
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.4 NaCl maximal stimulation at 0.15-0.2 M Drosophila sp. (in: flies)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.4 73000
-
 ncd polypeptide, SDS-PAGE Drosophila melanogaster
5.6.1.4 80000
-
 ncd Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Drosophila melanogaster
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Bos taurus
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Doryteuthis pealeii
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n Tetrahymena sp.
-
 ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Bos taurus
-
-
-
5.6.1.4 Bos taurus
-
 bovine
-
5.6.1.4 Doryteuthis pealeii
-
 squid
-
5.6.1.4 Drosophila melanogaster
-
-
-
5.6.1.4 Drosophila sp. (in: flies)
-
 recombinantly expressed in Escherichia coli
-
5.6.1.4 Tetrahymena sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.4 partial, recombinant protein, expressed in Escherichia coli Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Drosophila melanogaster
a
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Bos taurus
a
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Doryteuthis pealeii
a
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Tetrahymena sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.2 brain
-
 Bos taurus
-
5.6.1.4 brain
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
5.6.1.4 -80°C, 0.3 M NaCl, stored in liquid nitrogen Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.2 2',3'-dideoxy-ATP + H2O + a dynein associated with a microtubule at position n
-
 Bos taurus 2',3'-dideoxy-ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.2 2'-dATP + H2O + a dynein associated with a microtubule at position n
-
 Bos taurus 2'-dADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.2 3'-dATP + H2O + a dynein associated with a microtubule at position n
-
 Bos taurus 3'-dADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.2 ATP + H2O + a dynein associated with a microtubule at position n
-
 Bos taurus ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 2',3'-dideoxy-ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila sp. (in: flies) 2',3'-dideoxy-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 2'-dATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila melanogaster 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 2'-dATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila sp. (in: flies) 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 2'-dATP + H2O + a kinesin associated with a microtubule at position n
-
 Doryteuthis pealeii 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 3'-dATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila melanogaster 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 3'-dATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila sp. (in: flies) 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 3'-dATP + H2O + a kinesin associated with a microtubule at position n
-
 Doryteuthis pealeii 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila melanogaster ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila sp. (in: flies) ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Bos taurus ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Doryteuthis pealeii ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Tetrahymena sp. ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 additional information GTP is no substrate Drosophila melanogaster ?
-
 ?
5.6.1.4 additional information GTP is no substrate Doryteuthis pealeii ?
-
 ?
5.6.1.4 N6-methyl-ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila melanogaster N6-methyl-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
5.6.1.4 N6-methyl-ATP + H2O + a kinesin associated with a microtubule at position n
-
 Doryteuthis pealeii N6-methyl-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.1.4 dimer ncd polypeptide expressed in Escherichia coli Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
5.6.1.4 ncd
-
 Drosophila melanogaster
5.6.1.4 ncd
-
 Drosophila sp. (in: flies)
5.6.1.4 ncd
-
 Doryteuthis pealeii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.1.2 7
-
-
 Bos taurus
5.6.1.4 7
-
-
 Drosophila sp. (in: flies)
5.6.1.4 7
-
 ncd and cytoplasmic dynein Drosophila melanogaster