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Literature summary extracted from
- Binding sites on microtubules of kinesin motors of the same or opposite polarity (1996), Biochemistry, 35, 11203-11209.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.1.3 | expression in Escherichia coli | Drosophila sp. (in: flies) |
| 5.6.1.3 | KHC cloned and expressed in Escherichia coli BL21(DE3)pLysS | Drosophila sp. (in: flies) |
| 5.6.1.4 | motor domain construct MC6 is expressed in Escherichia coli | Drosophila sp. (in: flies) |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.1.4 | Kar3 | Ncd and Kar3, both minus-end motors, severely inhibit the binding of one another to microtubules | Drosophila sp. (in: flies) |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.6.1.3 | microtubule | binding site of plus-end-directed kinesin heavy chain (KHC) on microtubule | Drosophila sp. (in: flies) | 5874 | - |
| 5.6.1.4 | microtubule | binding-site of Ncd on microtubule | Drosophila sp. (in: flies) | 5874 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.6.1.3 | 37360 | - |
1 * 37360, KHC, molecular mass calculated from amino acid sequence predicted from nucleic acid sequence | Drosophila sp. (in: flies) |
| 5.6.1.3 | 90000 | - |
gel filtration | Drosophila sp. (in: flies) |
| 5.6.1.4 | 38910 | - |
Kar3, molecular mass calculated from the amino acid sequence predicted from the nucleic acid sequence | Saccharomyces cerevisiae |
| 5.6.1.4 | 41600 | - |
ncd, molecular mass calculated from the amino acid sequence predicted from the nucleic acid sequence | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.1.3 | ATP + H2O + a kinesin associated with a microtubule at position n | Drosophila sp. (in: flies) | - |
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? |  |
| 5.6.1.3 | ATP + H2O + a kinesin associated with a microtubule at position n | Xenopus laevis | - |
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| 5.6.1.3 | GTP + H2O + a kinesin associated with a microtubule at position n | Drosophila sp. (in: flies) | - |
GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| 5.6.1.3 | GTP + H2O + a kinesin associated with a microtubule at position n | Xenopus laevis | - |
GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | Drosophila melanogaster | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | Drosophila sp. (in: flies) | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | Saccharomyces cerevisiae | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | Xenopus laevis | - |
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.1.3 | Drosophila sp. (in: flies) | - |
- |
- |
| 5.6.1.3 | Xenopus laevis | - |
- |
- |
| 5.6.1.4 | Drosophila melanogaster | - |
- |
- |
| 5.6.1.4 | Drosophila sp. (in: flies) | - |
- |
- |
| 5.6.1.4 | Saccharomyces cerevisiae | - |
- |
- |
| 5.6.1.4 | Xenopus laevis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.1.3 | recombinant protein, expressed in Escherichia coli | Drosophila sp. (in: flies) |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.6.1.3 | ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) | microtubular motor protein, involved in organelle movement, in mitosis and meiosis. In contrast to dynein, it moves along microtubules towards the plus end. Composed of two heavy (a) chains (110kDa) and two more light (b) chains (65-75 kDa). Also hydrolyzes GTP. | Drosophila sp. (in: flies) | |
| 5.6.1.3 | ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) | microtubular motor protein, involved in organelle movement, in mitosis and meiosis. In contrast to dynein, it moves along microtubules towards the plus end. Composed of two heavy (a) chains (110kDa) and two more light (b) chains (65-75 kDa). Also hydrolyzes GTP. | Xenopus laevis | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules | Drosophila melanogaster | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules | Saccharomyces cerevisiae | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules | Xenopus laevis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.1.3 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila sp. (in: flies) | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| 5.6.1.3 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Xenopus laevis | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| 5.6.1.3 | GTP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila sp. (in: flies) | GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| 5.6.1.3 | GTP + H2O + a kinesin associated with a microtubule at position n | - |
Xenopus laevis | GDP + phosphate a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila melanogaster | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Drosophila sp. (in: flies) | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Saccharomyces cerevisiae | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| 5.6.1.4 | ATP + H2O + a kinesin associated with a microtubule at position n | - |
Xenopus laevis | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.6.1.3 | monomer | 1 * 37360, KHC, molecular mass calculated from amino acid sequence predicted from nucleic acid sequence | Drosophila sp. (in: flies) |
| 5.6.1.4 | monomer | - |
Drosophila melanogaster |
| 5.6.1.4 | monomer | - |
Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.1.3 | Eg5 | - |
Xenopus laevis |
| 5.6.1.3 | KHC | - |
Drosophila sp. (in: flies) |
| 5.6.1.4 | Kar3 | - |
Saccharomyces cerevisiae |
| 5.6.1.4 | minnus-end nonclaret disjunctional kinesin | - |
Drosophila sp. (in: flies) |
| 5.6.1.4 | minus-end nonclaret disjunctional kinesin | - |
Drosophila sp. (in: flies) |
| 5.6.1.4 | ncd | - |
Drosophila melanogaster |
| 5.6.1.4 | ncd | - |
Drosophila sp. (in: flies) |
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