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Literature summary extracted from
- Purification, cloning, and cofactor independence of glutamate racemase from Lactobacillus (1993), Biochemistry, 32, 3981-3990.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.3 | additional information | cofactor-independent enzyme | Limosilactobacillus fermentum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.3 | expression in Escherichia coli | Limosilactobacillus fermentum |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.1.1.3 | stabilized by presence of reduced thiols. After 3 h at 30°C, samples of the enzyme containing either dithiothreitol or Glu or both show about 80% retention of the original activity, whereas samples that lack both dithiothreitol and Glu retain less than 40% of the starting activity | Limosilactobacillus fermentum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.3 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Limosilactobacillus fermentum |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.3 | 0.24 | - |
L-Glu | - |
Limosilactobacillus fermentum | |
| 5.1.1.3 | 0.33 | - |
D-Glu | - |
Limosilactobacillus fermentum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 30000 | - |
gel filtration | Limosilactobacillus fermentum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.3 | Limosilactobacillus fermentum | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 158 | - |
enzyme from Lactobacillus fermenti | Limosilactobacillus fermentum |
| 5.1.1.3 | 161 | - |
enzyme expressed in Escherichia coli | Limosilactobacillus fermentum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.3 | D-Glu | - |
Limosilactobacillus fermentum | L-Glu | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.3 | monomer | 1 * 30000, SDS-PAGE | Limosilactobacillus fermentum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.3 | 69 | - |
D-Glu | - |
Limosilactobacillus fermentum | |
| 5.1.1.3 | 76 | - |
L-Glu | - |
Limosilactobacillus fermentum | |
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