BRENDA - Enzyme Database show

Plasmid-encoded resistance to arsenite and antimon

Kaur, P.; Rosen, B.P.; Plasmid 27, 29-40 (1992)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
G18D
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
7.3.2.7
G18R
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
7.3.2.7
G20S
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
7.3.2.7
G337R
mutation in ArsA protein, complete loss of resistance to the toxic oxyanions
Escherichia coli
7.3.2.7
K340E
retains about 10% of wild-type ATPase activity, no antimonite-stimulated ATPase activity
Escherichia coli
7.3.2.7
T22I
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.3.2.7
membrane
ArsA protein is an extrinsic membrane protein, ArsB protein is an integral membrane protein
Escherichia coli
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
Mg2+
required
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.3.2.7
Escherichia coli
-
-
-
7.3.2.7
Staphylococcus aureus
-
plasmid oI258
-
7.3.2.7
Staphylococcus xylosus
-
plasmid pSX267
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + antimonite/in
-
210288
Escherichia coli
ADP + phosphate + antimonite/out
-
210288
Escherichia coli
?
7.3.2.7
ATP + H2O + arsenite/in
-
210288
Staphylococcus aureus
ADP + phosphate + arsenite/out
-
210288
Staphylococcus aureus
?
7.3.2.7
ATP + H2O + arsenite/in
-
210288
Escherichia coli
ADP + phosphate + arsenite/out
-
210288
Escherichia coli
?
7.3.2.7
ATP + H2O + arsenite/in
-
210288
Staphylococcus xylosus
ADP + phosphate + arsenite/out
-
210288
Staphylococcus xylosus
?
7.3.2.7
additional information
The ArsA protein exhibits ATPase activity with a strict requirement for arsenite or antimonite. The binding of the ArsA protein to the ArsB protein on the inner membrane forms an active pump complex which catalyzes oxyanion transport, ArsB functions as the membrane anchor for the ArsA protein
210288
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
7.3.2.7
More
the ArsA is a 63000 Da protein, Ars B is a 45500 Da protein, ArsC is a 16000 Da, protein. ArsB, with 12 membrane-spanning segments forms the channel part and ArsA, occuring in pairs peripherally to the membrane
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
G18D
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
7.3.2.7
G18R
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
7.3.2.7
G20S
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
7.3.2.7
G337R
mutation in ArsA protein, complete loss of resistance to the toxic oxyanions
Escherichia coli
7.3.2.7
K340E
retains about 10% of wild-type ATPase activity, no antimonite-stimulated ATPase activity
Escherichia coli
7.3.2.7
T22I
mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.3.2.7
membrane
ArsA protein is an extrinsic membrane protein, ArsB protein is an integral membrane protein
Escherichia coli
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
Mg2+
required
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + antimonite/in
-
210288
Escherichia coli
ADP + phosphate + antimonite/out
-
210288
Escherichia coli
?
7.3.2.7
ATP + H2O + arsenite/in
-
210288
Staphylococcus aureus
ADP + phosphate + arsenite/out
-
210288
Staphylococcus aureus
?
7.3.2.7
ATP + H2O + arsenite/in
-
210288
Escherichia coli
ADP + phosphate + arsenite/out
-
210288
Escherichia coli
?
7.3.2.7
ATP + H2O + arsenite/in
-
210288
Staphylococcus xylosus
ADP + phosphate + arsenite/out
-
210288
Staphylococcus xylosus
?
7.3.2.7
additional information
The ArsA protein exhibits ATPase activity with a strict requirement for arsenite or antimonite. The binding of the ArsA protein to the ArsB protein on the inner membrane forms an active pump complex which catalyzes oxyanion transport, ArsB functions as the membrane anchor for the ArsA protein
210288
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.3.2.7
More
the ArsA is a 63000 Da protein, Ars B is a 45500 Da protein, ArsC is a 16000 Da, protein. ArsB, with 12 membrane-spanning segments forms the channel part and ArsA, occuring in pairs peripherally to the membrane
Escherichia coli