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Literature summary extracted from

  • Kaur, P.; Rosen, B.P.
    Plasmid-encoded resistance to arsenite and antimon (1992), Plasmid, 27, 29-40.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.7 G18D mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite Escherichia coli
7.3.2.7 G18R mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite Escherichia coli
7.3.2.7 G20S mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite Escherichia coli
7.3.2.7 G337R mutation in ArsA protein, complete loss of resistance to the toxic oxyanions Escherichia coli
7.3.2.7 K340E retains about 10% of wild-type ATPase activity, no antimonite-stimulated ATPase activity Escherichia coli
7.3.2.7 T22I mutation in ArsA protein, loss of resistance to the toxic oxyanions as well as inability to extrude arsenite Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.3.2.7 membrane ArsA protein is an extrinsic membrane protein, ArsB protein is an integral membrane protein Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.3.2.7 Mg2+ required Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.7 Escherichia coli
-
-
-
7.3.2.7 Staphylococcus aureus
-
 plasmid oI258
-
7.3.2.7 Staphylococcus xylosus
-
 plasmid pSX267
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.7 ATP + H2O + antimonite/in
-
 Escherichia coli ADP + phosphate + antimonite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in
-
 Staphylococcus aureus ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in
-
 Escherichia coli ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in
-
 Staphylococcus xylosus ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 additional information The ArsA protein exhibits ATPase activity with a strict requirement for arsenite or antimonite. The binding of the ArsA protein to the ArsB protein on the inner membrane forms an active pump complex which catalyzes oxyanion transport, ArsB functions as the membrane anchor for the ArsA protein Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.3.2.7 More the ArsA is a 63000 Da protein, Ars B is a 45500 Da protein, ArsC is a 16000 Da, protein. ArsB, with 12 membrane-spanning segments forms the channel part and ArsA, occuring in pairs peripherally to the membrane Escherichia coli