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Literature summary extracted from

  • Zhou, T.; Rosen, B.P.
    Asp45 is a Mg2+ ligand in the ArsA ATPase (1999), J. Biol. Chem., 274, 13854-13858.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.3.2.7 antimonite allosteric activation Escherichia coli
7.3.2.7 arsenite allosteric activation Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.7 D45A mutant of ArsA, the catalytic subunit of the pump. Inactive enzyme. ATP and Sb(III) synergistically protect wild type ArsA catalytic subunit from trypsin digestion. Subsequent addition of Mg2+ increases trypsin digestion. Mutant D45N and D45A remain protected by ATP and Sb(III) but lose the Mg2+ effect Escherichia coli
7.3.2.7 D45E approximately 5% of the wild type activity with about a 5fold decrease in affinity for Mg2+. ATP and Sb(III) synergistically protect wild type ArsA catalytic subunit from trypsin digestion. Subsequent addition of Mg2+ increases trypsin digestion, D45E exhibits an lower Mg2+ response Escherichia coli
7.3.2.7 D45N mutant of Ars A, the catalytic subunit of the pump. Inactive enzyme. ATP and Sb(III) synergistically protect wild type ArsA catalytic subunit from trypsin digestion. Subsequent addition of Mg2+ increases trypsin digestion. Mutant D45N and D45A remain protected by ATP and Sb(III) but lose the Mg2+ effect Escherichia coli

General Stability

EC Number General Stability Organism
7.3.2.7 ATP and Sb(III) synergistically protect wild type ArsA catalytic subunit from trypsin digestion. Subsequent addition of Mg2+ increases trypsin digestion. Mutant D45N and D45A remain protected by ATP and Sb(III) but lose the Mg2+ effect. D45E exhibits an intermediate Mg2+ response Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.3.2.7 Mg2+ required Escherichia coli
7.3.2.7 Mg2+ Asp45 of ArsA, the catalytic subunit of the pump is a Mg2+ ligand Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.7 ATP + H2O + arsenite/in Escherichia coli
-
ADP + phosphate + arsenite/out
-
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Organism

EC Number Organism UniProt Comment Textmining
7.3.2.7 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.7 ATP + H2O + arsenite/in
-
Escherichia coli ADP + phosphate + arsenite/out
-
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